Author/Authors :
Seyfi Abad Shapouri، M. نويسنده Faculty of Veterinary Medicine,Department of Pathobiology,Shahid Chamran University of Ahvaz,Ahvaz,Iran , , Ghiami Rad، M نويسنده Department of Genetics,Islamic Azad University, Ahar branch,Ahar,Iran , , Haji Hajikolaei، M.R. نويسنده Faculty of Veterinary Medicine,Department of Clinical Sciences,Shahid Chamran University of Ahvaz,Ahvaz,Iran , , Mahmoodi، P. نويسنده Faculty of Veterinary Science,Department of Pathobiology,Bu-Ali Sina University,Hamedan,Iran , , Karami، A. نويسنده Islamic Azad University, Ahar branch,Ahar,Iran , , Daghari، M. نويسنده Faculty of Veterinary Medicine,Department of Pathobiology,Shahid Chamran University of Ahvaz,Ahvaz,Iran ,
Abstract :
Bovine herpesvirus 1 (BoHV-1) is one of the most important infectious viruses of cattle which is considered as the causative agent of several clinical conditions in this species. Hence, we tried to isolate a local strain of the virus from carrier cattle in Ahvaz based on a strategy for reactivation of the latent infection in hosts having antibodies against the virus. Thus, 20 cattle were examined to identify seropositive animals using virus neutralization test (VNT). Afterward, 4 out of 8 seropositive cattle were selected and experimentally stressed by daily injection of Dexamethasone up to five days. Following the injection of Dexamethasone, nasal swabs were collected during 10 days and applied for virus isolation in cell culture. The results showed that one of the stressinduced animals shed the virus on days 8, 9 and 10 after injection of Dexamethasone. Identification of the reactivated virus was carried out based on the virusinduced cytopathic effects in cell culture and PCR and the results were confirmed by sequencing of the PCR product. Isolation of such a local strain of the virus can improve our understanding about circulating BoHV-1 strains in the country.
Keywords :
Virus reactivation , virus isolation , Ahvaz , BoHV-1 , Latent infection , dexamethasone
