Author/Authors :
Shavisi، N. نويسنده Department of Food Sciences and Technology, Faculty of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran , , Akhondzadeh Basti، A. نويسنده Professor of the Department of Food Hygiene, Faculty of Veterinary Medicine , , Khanjari، A. نويسنده Assistant Professor of the Department of Food Hygiene, Faculty of Veterinary Medicine , , Misaghi، A. نويسنده Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , , Shahbazi، Y. نويسنده , , Hajjar Bargh، A. نويسنده Department of Food Sciences and Technology, Faculty of Food Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran , , Vanaki، E. نويسنده Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran ,
Abstract :
Background: Innovative bioactive films incorporated with antimicrobial agents have
been recently developed for food preservation. The aim of this study was to evaluate
antibacterial activity of Polylactic Acid (PLA) films incorporated with Ziziphora
clinopodioides Essential Oil (ZEO) and Ethanolic Propolis Extract (EPE) against some
common food-borne pathogens.
Methods: The volatile chemical compounds of ZEO were identified by analytical gas
chromatography. Different PLA film groups were prepared using ZEO (1% and 2%) and
EPE (1% and 2%), separately and in combination. Disk diffusion method was used in
order to evaluate antibacterial activity of experimental groups. The analysis was
performed using SPSS 16.0.
Results: The PLA films enriched with the ZEO possess better antibacterial activity compared
to the films incorporated with EPE (p < 0.05). The descending order antibacterial
effects were as follows: Staphylococcus aureus > Listera monocytogenes > Bacillus
subtilis > B. cereus > Salmonella entritidis serovar Typhimurium > Escherichia coli
O157:H7. The combination of ZEO and EPE showed significantly higher antibacterial
effects against all bacteria than those of obtained with each single ZEO or EPE (p < 0.05).
Conclusion: The PLA films incorporated with ZEO and EPE had considerable antibacterial
activity, indicating potential of these films for application as active packaging in food
industry.
