Author/Authors :
Kouadio، I.K. نويسنده Food Microbiology and Biotechnology Laboratory, Nanguy Abrogoua University, Abidjan, Côte d’Ivoire , , Guessennd، N. نويسنده National Reference Center for Antibiotics, Pasteur Institute, Abidjan, Côte d’Ivoire , , Dadié، A. نويسنده Food Microbiology and Biotechnology Laboratory, Nanguy Abrogoua University, Abidjan, Côte d’Ivoire , , Gbonon، V. نويسنده National Reference Center for Antibiotics, Pasteur Institute, Abidjan, Côte d’Ivoire , , Tiékoura، B. نويسنده National Reference Center for Antibiotics, Pasteur Institute, Abidjan, Côte d’Ivoire , , Ouattara، M.B. نويسنده Food Microbiology and Biotechnology Laboratory, Nanguy Abrogoua University, Abidjan, Côte d’Ivoire , , Konan، F. نويسنده National Reference Center for Antibiotics, Pasteur Institute, Abidjan, Côte d’Ivoire , , Djè، K. M. نويسنده Science and Food Technology Unit, University Nangui Abrogoua , , Dosso، M. نويسنده National Reference Center for Antibiotics, Pasteur Institute, Abidjan, Côte d’Ivoire ,
Abstract :
Background: Enterococcus spp., belonging to the group of lactic acid bacteria, are
Gram-positive ubiquitous commensals of the intestines of human beings as well as warmblooded
animals. The main objective of this study was to determine the occurrence and
antimicrobial resistance of Enterococcus spp. isolated from lettuce and irrigation water in
Abidjan, Côte dʹIvoire.
Methods: A total of 72 samples, including leaves of lettuce (n=36) and irrigation water
(n=36) were randomly collected from three different agricultural sites located in Abidjan
city, Côte dʹIvoire. After microbial analysis and identification of Enterococcus spp. by
culturing and biochemical methods, antimicrobial susceptibility tests were carried out
using disk diffusion method. Data were analyzed by statistical processing software R (R
3.0 for Windows).
Results: E. faecalis was recognized as the most prevalent strain which was found in 27
out of 36 (75%) lettuce as well as 29 out of 36 (80.5%) irrigation water samples. The
mean Enterococcus load of lettuces and irrigation water samples were 2.3±0.7 and 3.6±2
log Colony Forming Unit per g lettuce, respectively. Among 45 studied enterococci
isolates, the most antibiotic-resistance rates were related to erythromycin (54%) and also
co-trimoxazole (49%).
Conclusion: There is a considerable public health concern regarding raw consumption of
lettuce cultivated in Abidjan city which can cause gastroenteritis diseases in consumers.
