Author/Authors :
Nilforoushzadeh، Mohammadali نويسنده Skin and Stem Cell Research Center, Tehran University of Medical Science, Tehran, Iran , , Rahimi Jameh، Elham نويسنده Skin and Stem Cell Research Center, Tehran University of Medical Science, Tehran, Iran , , Jaffary، Fariba نويسنده Skin and Stem Cell Research Center, Tehran University of Medical Science, Tehran, Iran , , Abolhasani، Ehsan نويسنده Skin Research center, Shohada-e-Tajrish Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Keshtmand، Gelavizh نويسنده Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran Keshtmand, Gelavizh , Zarkob، Hajar نويسنده Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran , , Mohammadi، Parvaneh نويسنده Gastroenterology and liver diseases Research center, Shahid Beheshti University of Medical Sciences, Tehran,. , , Aghdami، Nasser نويسنده Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran ,
Abstract :
Objective: Dermal papilla and hair epithelial stem cells regulate hair formation and
the growth cycle. Damage to or loss of these cells can cause hair loss. Although
several studies claim to reconstitute hairs using rodent cells in an animal model,
additional research is needed to develop a stable human hair follicle reconstitution
protocol. In this study, we have evaluated hair induction by injecting adult cultured
human dermal papilla cells and a mixture of hair epithelial and dermal papilla cells in
a mouse model.
Materials and Methods: In this experimental study, discarded human scalp skins were
used to obtain dermal papilla and hair epithelial cells. After separation, cells were cultured
and assessed for their characteristics. We randomly allocated 15 C57BL/6 nude mice into
three groups that received injections in their dorsal skin. The first group received cultured
dermal papilla cells, the second group received a mixture of cultured epithelial and dermal
papilla cells, and the third group (control) received a placebo [phosphate-buffered saline
(PBS-)].
Results: Histopathologic examination of the injection sites showed evidence of hair
growth in samples that received cells compared with the control group. However, the
group that received epithelial and dermal papilla cells had visible evidence of hair growth.
PKH tracing confirmed the presence of transplanted cells in the new hair.
Conclusion: Our data showed that injection of a combination of adult human cultured
dermal papilla and epithelial cells could induce hair growth in nude mice. This study emphasized
that the combination of human adult cultured dermal papilla and epithelial cells
could induce new hair in nude mice.
