Title of article :
Growth Inhibitory Effect of Anthemis haussknechtii Root Extract, as a Source of Parthenolide, on Breast Cancer Cell Line
Author/Authors :
Jafari، Nesa نويسنده Department of Energy and Environmental Biotechnology,National Institute of Genetic Engineering and Biotechnology,Tehran,Iran , , Nazeri، Sonbol نويسنده Department of Plant Biotechnology,Bu-Ali Sina University,Hamedan,Iran , , Rabiei، Zohreh نويسنده Department of Energy and Environmental Biotechnology,National Institute of Genetic Engineering and Biotechnology,Tehran,Iran , , Tahmasebi Enferadi، Sattar نويسنده Department of Energy and Environmental Biotechnology,National Institute of Genetic Engineering and Biotechnology,Tehran,Iran , , Behroozi، Reza نويسنده Department of Energy and Environmental Biotechnology,National Institute of Genetic Engineering and Biotechnology,Tehran,Iran ,
Abstract :
Parthenolide is major Sesquiterpene lactones present in Anthemis haussknechtii Boiss. & Reut. (feverfew). This compound has many effects on different disease such as migrain and cancer. Parthenolide was reported from Tanacetum parthenium (L.) Sch.Bip. but other plants of Asteraceae family could contain parthenolide. In this study parthenolide was extracted and identified with two methods, Fourier Transform Infra-Red Spectroscopy (FTIR) and High Performance Liquid Chromatography (HPLC). Breast cancer cell line, MDA-MB-231, was exposed to different concentrations of parthenolide for 24 hours. Half maximal inhibitory concentration (IC50) was evaluated using Methylthiazol Tetrazolium (MTT) test. Based on results, 1000 µg/ml concentration is the minimum lethal dose that kills approximately 50% of cells after 24 hours. The results revealed that the A. haussknechtii parthenolide dramatically decreased survival of cancer cell line by inducing apoptosis. This is the first report of cytotoxicity effect of A. haussknechtii extract on breast cancer cell line.
Keywords :
Cell viability , FT-IR; HPLC , MTT test , Parthenolide
