Author/Authors :
Talazadeh، Forough نويسنده 1Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2Professor, Department , , Mayahi، Mansour نويسنده 2Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran , , Nofouzi، Katayoon نويسنده , , Golzari، Ebrahim نويسنده 4Graduate from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran4Graduate from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran4Graduate from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran , , Chegini، Rahil نويسنده 4Graduate from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran ,
Abstract :
Background: In poultry production, improving immunity is very important in preventing infectious diseases. One solution to improve the immunity of animals and to decrease their susceptibility to infectious diseases is administration of immunostimulants. Surveys have indicated that some bacteria such as Mycobacterium vaccae can work as immunomodulators, promote Th1-mediated mechanisms, and switch off pre-existing Th2 preponderance.1
Objectives: The aim of this study was to examine the effect of Tsukamurella inchonensis bacterin on the immune response against influenza and Newcastle disease (ND) vaccines in broiler chickens.
Materials and Methods: A total of 170 one-day-old broiler chicks were purchased and divided randomly into 5 equal groups. Chickens of group A received 106 bacterin subcutaneously 2 days before vaccination against ND and avian influenza (AI). Chickens of group B received 106 bacterin subcutaneously 6 days after the first injection of bacterin. Chickens of group C received 106 bacterin subcutaneously 6 days after the second injection of bacterin. Chickens of group D were vaccinated against ND and AI but did not receive bacterin. Chickens of group E were not vaccinated against ND and AI and did not receive bacterin. All groups except group E were vaccinated with live Newcastle vaccine and AI-ND killed vaccine (subtype H9N2). Blood samples were collected and antibody titer against ND vaccine and AI vaccine was determined by hemagglutination inhibition (HI) test.
Results: The results of present study showed that receiving T. inchonensis bacterin for 3 times significantly increased the specific antibody response to AI subtype H9N2 vaccine. Moreover, the specific antibody response to Newcastle vaccine was significantly increased at days 21 and 28 after vaccination.
Conclusion: Receiving T. inchonensis bacterin can enhance immune response against ND virus and AI virus.
