Author/Authors :
Pastrelo، Maurício Mercaldi نويسنده Department of Pathology,Universidade Federal de São Paulo,São Paulo,Brazil , , Ribeiro، Carla Caroline Dias نويسنده Department of Medicine,Universidade Federal de São Paulo,São Paulo,Brazil , , Duarte، Joselmo Willamys نويسنده Department of Pathology,Universidade Federal de São Paulo,São Paulo,Brazil , , Gollucke، Andréa Pitelli Bioago نويسنده Department of Biosciences,Universidade Federal de São Paulo,Santos,Brazil , , Artigiani-Neto، Ricardo نويسنده Department of Pathology,Universidade Federal de São Paulo,São Paulo,Brazil , , Ribeiro، Daniel Araki نويسنده Department of Pathology, Department of Biosciences,Universidade Federal de São Paulo,São Paulo,Brazil , , Miszputen، Sender Jankiel نويسنده Department of Medicine,Universidade Federal de São Paulo,São Paulo,Brazil , , Oshima، Celina Tizuko Fujiyama نويسنده Department of Pathology,Universidade Federal de São Paulo,São Paulo,Brazil , , Paiotti، Ana Paula Ribeiro نويسنده Department of Pathology, Department of Medicine,Universidade Federal de São Paulo,São Paulo,Brazil ,
Abstract :
Reactive oxygen and nitrogen species (ROS/RNS) play a crucial role in inflammatory bowel disease (IBD) exacerbating the chronic inflammatory process. Endogenous and diet antioxidants can neutralize these compounds. The apple is widely consumed, with several antioxidant activity compounds. The present study evaluated the effects of concentrated apple extract (CAE) in acetic acid induced colitis. 29 Wistar male rats were randomized into 5 groups. G1–Sham/saline solution, G2–CAE/control, G3–acetic acid/control, G4–curative- CAE treatment and G5–preventive-CAE treatment. Eight days later, the animals were euthanized and the colonic segment resected for macroscopic and histological analysis. Gene expression was evaluated for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), catalase and copper and zinc superoxide dismutase (CuZnSOD) by quantitative real time PCR, while protein expression was assessed for iNOS, COX-2 and 8- hydroxy-20-deoxyguanosine (8-OHdG) via immunohistochemistry. The groups G3, G4 and G5 had weight loss, while G5 had weight increase at the end of the experiment. The treatment with CAE reduced the macroscopic and microscopic injury, decreased iNOS mRNA expression and increased CuZnSOD mRNA expression in animals with induced acetic acid-colitis. The findings of the present study suggest that CAE treatment exerts an antioxidant role by downregulating iNOS and upregulating CuZnSOD.
Keywords :
iNOS , CuZnSOD , antioxidant , Apple extract , experimental colitis
