Author/Authors :
Taghavi Mohammad-Mohsen نويسنده Department of Anatomy, Social Determinants of Health Research Centre, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Taghavi Mohammad-Mohsen , Shabanizadeh Ahmad نويسنده Department of Anatomy, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran , Taghipour Zahra نويسنده Department of Anatomy, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran , Salahshoor Mohammad Reza نويسنده Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran , Jalili Cyrus نويسنده Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran , Shariati Kohbanani Mahdi نويسنده PhD in Anatomy, Department of Anatomy Shariati Kohbanani Mahdi , Jafarinaveh Hamidreza نويسنده Department of Anatomy, Medical School, Rafsanjan
University of Medical Sciences, Rafsanjan, IR Iran , Mollahosseni Akram نويسنده Department of Anatomy, Medical School, Rafsanjan
University of Medical Sciences, Rafsanjan, IR Iran
Abstract :
Background MTHFR gene is one of the main and effective factors in
genes methylation. This gene has a common polymorphism in cordon 677,
which can affect its activity. Changing activity rate of this gene can
affect methylation rate of tumor suppressor genes and cell membrane
proteins and other genes in addition to their expression rate.
Therefore, it can be considered as one of the effective factors on
producing cancer. Methods In this descriptive-cross sectional study, 34
cancer and non-cancer tissue samples were studied. Initially, DNA was
extracted from samples and then E-cadherin and MTHFR c677t polymorphism
were amplified by the polymerase chain reaction (PCR). Methylation
status of E-cadherin was evaluated by adding methylation restriction
enzyme HpaII and polymorphism of MTHFR c677t was assessment by the
restriction fragment length polymorphism (RFLP) method. Results
Methylation status of E-cadherin gene numbers of methylated cases in the
cancer group were equal to one and unmethylated cases equal to 33 and
numbers of unmethylated cases in the non-cancer group was equal to 34
while there were no methylated cases. In assessment of methylation
status of E-cadherin and different genotypes of MTHFR in codon number
677 in cancer samples, number of CC, CT and TT genotypes were equal to
0, 1 and 0 in the methylated group and equal to 6, 27 and 1 in the
unmethylated group, respectively. Conclusions According to this
observation, another factor such as oncogenes activity may cause cancer
in samples but CT genotype of MTHFR can be considered as an effective
factor in creating cancer.
