Author/Authors :
Zandi Mohammad نويسنده Department of Animal and Poultry Science and Fisheries, Agricultural Institute, Iranian Research Organization for Science and Technology, , Masoumian Majid نويسنده Department of Agriculture, Iranian Research Organization
for Science and Technology (IROST), Tehran, IR Iran , Shariatinia Asghar نويسنده Department of Agriculture, Iranian Research Organization
for Science and Technology (IROST), Tehran, IR Iran , Sanjabi Mohammad Rez نويسنده Department of Agriculture, Iranian Research Organization
for Science and Technology (IROST), Tehran, IR Iran
Abstract :
Background Many studies examine the antibacterial effects of
medicinal plants; however, little research is done to evaluate their
effects on different cell types, especially dermal fibroblasts.
Objectives The current study aimed to study the effect of different
concentrations of Aloe Vera, henna, chamomile, myrtle, mint, licorice,
cinnamon, ginger and cedar extracts and their synergistic effects on the
viability of dermal fibroblasts. Methods To evaluate the performance of
herbal extracts on dermal fibroblasts, in the first experiment different
concentrations (6.25, 12.5, 25, 50, 100, 250, 500 and 1000 µg/mL) of the
extracts were evaluated by the MTT cell proliferation assay. In the
second experiment, the minimum effective concentrations of the plant
extracts in triple combination were evaluated in the cells under study.
Results The minimum effective concentrations of henna, chamomile,
myrtle, mint, cinnamon, ginger and cedar were 12.5, 6.25, 6.25, 6.25,
6.25, 12.5 and 12.5µg/mL, respectively. Results showed that, by
comparing the minimum effective concentration of herbal extracts, the
viability of dermal fibroblasts significantly increased by cedar extract
(P < 0.05). Combination of Aloe Vera, licorice and mint extracts
significantly increased the viability of dermal fibroblasts (P <
0.05). Conclusions Based on the results of the current study, it was
concluded that Aloe vera, licorice and mint extracts had synergistic
effects on the viability of dermal fibroblasts; in addition, the
combination of Aloe vera and licorice with either henna or myrtle, and
Aloe vera and mint with either cedar or ginger resulted in synergistic
effects on viability of dermal fibroblasts. The third category of triple
combinations of herbal extracts with synergistic effects on the cells
under study was the combination of Aloe Vera and mint with either
chamomile or cinnamon and also Aloe vera and licorice with either myrtle
or cedar.
