Author/Authors :
Mohammadi, Hadi Department of Chemistry - Islamic Azad University North Tehran Branch Iran , Abedi, Anita Department of Chemistry - Islamic Azad University North Tehran Branch Iran , Akbarzadeh, Azim Department of Pilot Nanobiotechnology - Pasteur Institute of Iran , Mokhtari, Mohammad Javad Department of Biology - Islamic Azad University Zarghan Branch Iran , Ebrahimi Shahmabadi, Hasan Department of Chemistry - Islamic Azad University North Tehran Branch Iran , Mehrabi, Mohamad Reza Department of Chemistry - Islamic Azad University North Tehran Branch Iran , Javadian, Saifuddin Department of Biochemistry - Pasteur Institute of Iran Tehran , Chiani, Mohsen Department of Chemistry - Islamic Azad University North Tehran Branch Iran
Abstract :
Platinum nanoparticles (PNPs) were synthesized by chemical reduction of potassium hexachloroplatinate (IV) with trisodium citrate under vigorous stirring and addition of sodium dodecyl sulfate as stabilizer reagent. Reducing agent was chosen depending on the oxidation reactions and potential values of the chemical materials used in the experiment. The aim of this study is to investigate the effects of PNPs on the different cancer cell lines and cytotoxicity study of this nanomaterial. The morphology of PNPs was investigated by scanning electron microscope (XL30, Philips Electronics, Amsterdam, The Netherlands) with the ability to perform elemental analysis by EDX. Malvern Zetasizer 3000 HSA (Malvern Instruments, Worcestershire, UK) was used to determine the distribution of particle size and zeta potential of PNPs. The cytotoxicity property of the nanoparticles was evaluated by MTT assay on MCF-7 and HepG-2 cell lines, and the cytotoxic concentration 50% values were determined for 24 h.
Keywords :
Platinum nanoparticles , Potassium hexachloroplatinate , Chemical reduction , MTT assay MCF-7 , HepG-2
