Author/Authors :
Yang, Xiao-Lu School of Life Sciences - Henan University, Kaifeng, China , Zhang, Yu-Shuai School of Life Sciences - Henan University, Kaifeng, China , Yang, Tian School of Life Sciences - Henan University, Kaifeng, China , Geng, Fang-Yong School of Life Sciences - Henan University, Kaifeng, China , Li, Di School of Life Sciences - Henan University, Kaifeng, China , Xiao, Bao-Lin School of Life Sciences - Henan University, Kaifeng, China , Hong, Jun School of Life Sciences - Henan University, Kaifeng, China , Moosavi-Movahedi, Ali Akbar Institute of Biochemistry and Biophysics - University of Tehran, Tehran, Iran , Ghourchian, Hedayatollah Institute of Biochemistry and Biophysics - University of Tehran, Tehran, Iran
Abstract :
A high catalytic efficient soft-template nanostructured peroxidase (SP) was constructed when cytochrome c (Cyt c, 10 μM) was mixed with sodium decyl sulfate (SDeS, 40 mM) in 100 mM sodium phosphate buffer solution (PBS). The electrochemical investigations were carried out for the SP immobilized on hydroxyl fullerenes modified glassy carbon electrode (GCE). The cyclic voltammetry of the SP-modified GCE showed a pair of well-defined redox peaks with a formal potential (E∘′) of −220 ± 2 mV (vs. Ag/AgCl) in 100 mM, pH 8.0 PBS at a scan rate of 0.05 Vs−1. The heterogeneous electron transfer rate constant (k s ) was calculated to be 14.3 ± 1 s−1. The apparent Michaelis–Menten constant (Kappm) was 1.79 ± 0.03 µM. SDeS and Cyt c may be important for the structure and catalytic function of SP, respectively. The SP-modified GCE could be used as an H2O2 biosensor instead of horseradish peroxidase (HRP), with good sensitivity and stability.
Keywords :
Cytochrome c , Sodium decyl sulfate , Soft-template nanostructured peroxidase , Hydroxyl fullerenes , Electrochemistry
