Author/Authors :
Mortazavi, Mohammad Javad Ionizing and Non-ionizing Radiation Protection Research Center , Shekoohi-Shooli, Fatemeh Radiology and Radiotherapy Department - Shahid Beheshti University of Medical Science, Tehran, Iran , Aghamir, Mahmood Reza Radiology and Radiotherapy Department - Shahid Beheshti University of Medical Science, Tehran, Iran , Mehrabani, Davood Regenerative Medicine Department - University of Manitoba, Winnipeg, Manitoba, Canada , Dehghanian, Amirreza Department of Pathology - School of Medicine - Trauma Research Center - Shahid Rajayee Hospital , Zare, Shahrokh Stem Cell and Transgenic Technology Research Center , Mosleh-Shirazi, Mohammad Amin Shiraz University of Medical Sciences, Shiraz, Iran
Abstract :
Objectives: To determine the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on
regeneration of bone marrow and intestinal tissue and survival rate in experimental mice with acute
radiation syndrome (ARS).
Methods: Forty mice were randomly divided into two equal groups of A receiving no BMSC transplantation
and B receiving BMSCs. BMSCs were isolated from the bone marrow and cultured in DMEM media. Both
groups were irradiated with 10 Gy (dose rate 0.28 Gy/ min) 60CO during 35 minutes with a field size of
35×35 for all the body area. Twenty-four hours after γ irradiation, 150×103 cells of passage 5 in 150 μl
medium were injected intravenously into the tail. Animals were euthanized one and two weeks after cell
transplantation. They were evaluated histologically for any changes in bone marrow and intestinal tissues.
The survival rate in mice were also determined.
Results: A significant increase for bone marrow cell count and survival rate were observed in group B in
comparison to group A. Histological findings denoted to a healing in sample tissues.
Conclusion: BMSCs could significantly reduce the side effects of ARS and increase the survival rate and
healing in injured tissue. As such their transplantation may open a window in treatment of patients with
ARS.
Keywords :
Acute radiation syndrome , Mesenchymal stem cell , Bone marrow , Healing , Survival , Mouse
