Author/Authors :
Saraswat, S Physiology Reproduction and Shelter Management (PRSM) Division - Central Institute for Research on Goats (CIRG), Mathura, Uttar Pradesh, India , Rout, P. K GGB Division - Central Institute for Research on goats (CIRG), Mathura, Uttar Pradesh, India , Kharche, S. D Physiology Reproduction and Shelter Management (PRSM) Division - Central Institute for Research on Goats (CIRG), Mathura, Uttar Pradesh, India , Goel, A. K Physiology Reproduction and Shelter Management (PRSM) Division - Central Institute for Research on Goats (CIRG), Mathura, Uttar Pradesh, India , Jindal, S. K Physiology Reproduction and Shelter Management (PRSM) Division - Central Institute for Research on Goats (CIRG), Mathura, Uttar Pradesh, India , Kumar, S Physiology Reproduction and Shelter Management (PRSM) Division - Central Institute for Research on Goats (CIRG), Mathura, Uttar Pradesh, India
Abstract :
The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile
and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol
method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression
pattern of ESR1 gene was analyzed by RT-PCR (Roche LC-480). Relative quantification by RT-PCR indicated that the ESR1 gene
expression showed more fold in fertile bucks as compared to non-fertile.
Farsi abstract :
مطالعه حاضر به منظور تجزيه و تحليل الگوي بيان ژن گيرنده استروژني 1 (ESR1) در بزهاي نر بربري (بارور و نابارور) بر اساس صفات كيفي مايع مني و صفات باروري انجام شد. RNA به روش Trizol از طحال استخراج شد. الگوي بيان ژن ESR1 با استفاده از Real Time PCR (Roche LC-480) مورد تجزيه و تحليل قرار گرفت. تعيين ميزان نسبي با RT-PCR نشان داد كه بيان ژن ESR1 در بزهاي بارور در مقايسه با نابارورها بيشتر است.
Keywords :
ESR1 gene , Estrogen receptors , RT-PCR , RNA
