• Title of article

    Rapid Etiologic Diagnosis of Herpes Virus Infections in Children

  • Author/Authors

    Li Wei نويسنده , Liu Yujie نويسنده , Wu Yidong نويسنده Yunnan University , Shen Xiaoting نويسنده Department of Clinical Laboratory, Children’s Hospital of Zhejiang University School of Medicine, Hangzhou 310003, PR China , Gao Huihui نويسنده Department of Clinical Laboratory, Children’s Hospital of Zhejiang University School of Medicine, Hangzhou 310003, PR China , Shang Shiqiang نويسنده Department of Clinical Laboratory, Children’s Hospital of Zhejiang University School of Medicine, Hangzhou 310003, PR China

  • Pages
    6
  • From page
    1
  • Abstract
    Background Human herpes viruses, as common viruses, not only affect mainly the skin, mucosa, and nervous tissue, but also can cause a variety of serious diseases in children. Objectives The aim of this study was to determine the sensitivity and specificity of multiplex PCR-based DNA microarray technology in comparison with PCR method and IgM ELISA. Methods A total of 108 blood samples from children with viral infections were collected and analyzed by multiplex PCR-based DNA microarray technology, PCR method, and IgM ELISA. Results Of 108 specimens, 16 were positive which gave a positive rate of 14.8%. Most of the patients were infected with EBV and HCMV. The sensitivity and specificity of this technology for detecting human herpes viruses were 100% when compared to PCR method. The crude agreement between multiplex PCR-based DNA microarray technology and IgM ELISA for detecting human herpes viruses was 95.4%. Conclusions The results indicated that multiplex PCR-based DNA microarray technology is a rapid auxiliary diagnostic method for simultaneous detection of the seven common herpes viruses with high sensitivity and specificity.
  • Journal title
    Astroparticle Physics
  • Serial Year
    2017
  • Record number

    2407653

    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=10&DC=2407653