Author/Authors :
Li Wei نويسنده , Liu Yujie نويسنده , Wu Yidong نويسنده Yunnan University , Shen Xiaoting نويسنده Department of Clinical Laboratory, Children’s Hospital of
Zhejiang University School of Medicine, Hangzhou 310003, PR
China , Gao Huihui نويسنده Department of Clinical Laboratory, Children’s Hospital of
Zhejiang University School of Medicine, Hangzhou 310003, PR
China , Shang Shiqiang نويسنده Department of Clinical Laboratory, Children’s Hospital of
Zhejiang University School of Medicine, Hangzhou 310003, PR
China
Abstract :
Background Human herpes viruses, as common viruses, not only
affect mainly the skin, mucosa, and nervous tissue, but also can cause a
variety of serious diseases in children. Objectives The aim of this
study was to determine the sensitivity and specificity of multiplex
PCR-based DNA microarray technology in comparison with PCR method and
IgM ELISA. Methods A total of 108 blood samples from children with viral
infections were collected and analyzed by multiplex PCR-based DNA
microarray technology, PCR method, and IgM ELISA. Results Of 108
specimens, 16 were positive which gave a positive rate of 14.8%. Most of
the patients were infected with EBV and HCMV. The sensitivity and
specificity of this technology for detecting human herpes viruses were
100% when compared to PCR method. The crude agreement between multiplex
PCR-based DNA microarray technology and IgM ELISA for detecting human
herpes viruses was 95.4%. Conclusions The results indicated that
multiplex PCR-based DNA microarray technology is a rapid auxiliary
diagnostic method for simultaneous detection of the seven common herpes
viruses with high sensitivity and specificity.
