Author/Authors :
Chen Min نويسنده , Xue Heng نويسنده Department of Laboratory Medicine, Fujian Provincial
Maternity and Children Hospital, Affiliated Hospital of Fujian
Medical University, Fuzhou, China , Xue Xiaoguang نويسنده Department of Laboratory Medicine, Fujian Provincial
Maternity and Children Hospital, Affiliated Hospital of Fujian
Medical University, Fuzhou, China , Yang Changyi نويسنده Department of Neonatology, Fujian Provincial Maternity and
Children Hospital, Affiliated Hospital of Fujian Medical University,
Fuzhou, China , Chen Qingquan نويسنده Department of Laboratory Medicine, Medical Technology and
Engineering College, Fujian Medical University, Fuzhou,
China , Lin Ni نويسنده Department of Laboratory Medicine, Medical Technology and
Engineering College, Fujian Medical University, Fuzhou,
China , Lin Yujun نويسنده The fourth ward, Fuzhou Tuberculosis Prevention and Cure
Hospital, Fuzhou, China , Rao Dongdong نويسنده Department of Laboratory Medicine, Fujian Provincial
Maternity and Children Hospital, Affiliated Hospital of Fujian
Medical University, Fuzhou, China , Lin Huizi نويسنده Department of Neonatology, Fujian Provincial Maternity and
Children Hospital, Affiliated Hospital of Fujian Medical University,
Fuzhou, China
Abstract :
Background The diagnosis of neonatal sepsis remains a challenge as
the condition lacks early clinical signs and reliable biomarkers.
Objectives The aim of our study was to determine whether serum mannose
binding lectin (MBL) levels and genotypes of MBL2
gene could be used as markers for predicting neonatal sepsis in the
Chinese Han population. Methods This prospective study was
hospital-based in design. 48 neonates with clinical signs and symptoms
of septicemia (study group), and 96 infants with no infection (control)
were included. All the neonates are Chinese Han descent.
MBL2 promoter polymorphisms at positions -550, -221
and +4 were analyzed by direct sequencing, and serum MBL levels were
estimated by enzyme-linked immunesorbent assay. Results Frequencies of
genotype -221 YX were significantly higher in the study group (45.8%)
compared with the control group (15.60%; P = 0.00009). The median serum
MBL level was found to be significantly lower in infants who had the
-221YX genotype (214.54 ng/mL) compared with those who had the -221YY
genotype (597.85 ng/mL; P < 0.0001). Additionally, the median of
serum MBL was significantly lower in infants with septicemia (289.65
ng/mL) than in controls (597.75 ng/mL; P = 0.041). According to ROC
analysis, the cutoff value of MBL concentration ≤ 384.60 (ng/mL) had a
sensitivity of 0.806 and a specificity of 0.777 for predicting sepsis.
Conclusions This study suggested that serum MBL and the -221 YX genotype
of the MBL2 gene might be predisposed factors for
sepsis in the Chinese Han population.
