Author/Authors :
Motamedifar Mohammad نويسنده Department of Bacteriology and Virology, Shiraz HIV/AIDS Research Center (SHARC), Shiraz University of Medical Sciences, Shiraz, Iran , Safaei Akbar نويسنده Department of Pathology, Shahid Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran , Sarvari Jamal نويسنده Department of Bacteriology and Virology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran , Zamani Khosrow نويسنده Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran , Khashei Reza نويسنده Department of Bacteriology and Virology, School of
Medicine, Shiraz University of Medical Sciences, Shiraz, IR
Iran , Mahmoudvand Shahab نويسنده Department of Bacteriology & Virology, School of
Medicine, Shiraz University of Medical Sciences, Shiraz,
Iran , Azizi Zohre نويسنده Department of Bacteriology & Virology, School of
Medicine, Shiraz University of Medical Sciences, Shiraz,
Iran
Abstract :
Background Worldwide, colorectal cancer is the fourth most common
cause of cancer-related deaths. Infectious agents have long been
associated with development of gastrointestinal malignancies including
colorectal cancer. Therefore, the aim of this study was to detect
Streptococcus gallolyticus subsp.
gallolyticus and Helicobacter
pylori in colorectal cancer tissue specimens in comparison
with healthy tissue specimens. Methods A total of 210 tissue samples
including 70 adenocarcinoma colorectal tissue, 70 adenomatous polyposis
colorectal tissues, and 70 normal colorectal tissues were subjected to
DNA extraction. The quality of the extracted DNA was confirmed by the
amplification of a β-globin fragment using polymerase chain reaction
(PCR). The presence of sod and glm
genes were evaluated as Streptococcus gallolyticus
and H. pylori presence markers by PCR method,
respectively. Results Out of 210 subjects, 112 were male and the rest
were female. The age of our patients ranged from 22 to 87 with an
average of 54 years. None of the samples in two studied groups were
positive for the sod and glm
genes. Conclusions According to our results, S.
gallolyticus subsp. gallolyticus and
H. pylori might not be involved in colorectal cancer
pathogenesis. More investigation on huge sample in different area might
be clarified this results.
