Author/Authors :
Besharat Sima نويسنده Department of Obstetrics and Gynecology, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran , Poustchi Hossein نويسنده Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran Poustchi Hossein , Montazeri Ghodratollah نويسنده Digestive Disease Research Centre, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran , Mohamadkhani Ashraf نويسنده Digestive Disease Research Centre, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran , Sharafkhah Maryam نويسنده Department of Epidemiology and Biostatistics, Tehran University of Medical Sciences, Tehran, Iran. Sharafkhah Maryam , Mirzaei Samaneh نويسنده , Moafi Samaneh نويسنده Liver and Pancreatobiliary Diseases Research Center,
Digestive Diseases Research Institute, Tehran University of Medical
Sciences, Tehran, IR Iran , Fazli Hamid Reza نويسنده Liver and Pancreatobiliary Diseases Research Center,
Digestive Diseases Research Institute, Tehran University of Medical
Sciences, Tehran, IR Iran
Abstract :
Background Chronic hepatitis B (CHB), with accumulation of
Hepatitis B surface Antigen (HBsAg) in hepatocytes, linked to the
immune-mediated hepatic inflammation and induction of oxidative stress.
8-Hydroxyl-2ʹ-deoxyguanosine (8-OHdG) is a useful biomarker for
measuring the adverse effects of exogenous infectious agents in
oxidative damage to DNA. Objectives The current study aimed at
investigating the possible oxidative adverse effects of HBsAg and
systemic DNA damage in patients with CHB, and supporting the host-viral
interaction in immune-mediated inflammatory. Methods Thirty patients
with CHB who had undergone liver biopsies for therapeutic purposes and
30 matched controls from a healthy population were randomly selected in
the present study for assessment of 8-OHdG levels in peripheral blood
leukocytes DNA by 32P-postlabeling analysis. Expression of HBsAg in
hepatocytes was evaluated immunohistochemically in liver biopsies of
patients with CHB. The effect of 8-OHdG and 95% confidence interval
(CI), adjusted by relevant confounders, were assessed on hepatitis B
virus (HBV) infection. Results Experimental investigation showed
increased levels of DNA adduct 8-OHdG compared with healthy individuals
(mean ± standard deviation, 1456 ± 1275 vs. 402 ± 271; P <
0.001). The logistic regression with continuous and dichotomous models
revealed the strong impact of 8-OHdG on CHB infection (OR = 1.20; 95%CI:
1.01 - 1.44, P = 0.043) and (OR = 7.18; 95%CI: 1.32 - 39.02, P = 0.022).
HBV DNA and hepatic expression of HBsAg had a borderline association
with DNA adduct 8-OHdG (r = 0.35, P = 0.054 and r = 0.36, P = 0.05).
Conclusions The current study showed that the adduct of 8-OHdG in
peripheral blood cells DNA increased in patients with CHB compared with
healthy carriers and the pathophysiologic role of HBsAg in oxidative
stress in patients with CHB. Nonetheless, the lack of efficient DNA
repair enzymes activity as well as a proper diet with antioxidant agents
in CHB need to be clarified in future studies.
