Author/Authors :
Jiang Tian-Su نويسنده , Yan Jianxin نويسنده Department of Laboratory Medicine, the First People’s
Hospital of Wenling, Taizhou, China , Liang Jinhua نويسنده Department of Laboratory Medicine, Hongqi Affiliated
Hospital of Mudanjiang Medical College, Mudanjiang,
China , Lv Tao نويسنده State Key Laboratory for Diagnosis and Treatment of
Infectious Diseases, The First Affiliated Hospital, School of
Medicine, Zhejiang University , Gu Silan نويسنده State Key Laboratory for Diagnosis and Treatment of
Infectious Diseases, The First Affiliated Hospital, School of
Medicine, Zhejiang University , Huang Linyao نويسنده Department of Laboratory Medicine, the First People’s
Hospital of Wenling, Taizhou, China , Shen Ping نويسنده State Key Laboratory for Diagnosis and Treatment of
Infectious Diseases, The First Affiliated Hospital, School of
Medicine, Zhejiang University , Fang Yunhui نويسنده State Key Laboratory for Diagnosis and Treatment of
Infectious Diseases, The First Affiliated Hospital, School of
Medicine, Zhejiang University , Chen Yunbo نويسنده State Key Laboratory for Diagnosis and Treatment of
Infectious Diseases, The First Affiliated Hospital, School of
Medicine, Zhejiang University
Abstract :
Background The incidence of Clostridium difficile
infection (CDI) has markedly increased over the past decade.
Although its epidemiology has been previously investigated in tertiary
hospitals, no studies have investigated the prevalence of CDI in county
level hospitals in China. Objectives This study aimed at describing the
molecular characteristics of toxigenic C. difficile
isolated from a community level hospital and evaluating physicians’
knowledge on CDI. Methods We conducted a 15-month study at a country
level hospital to characterize clinical isolates of C.
difficile. A total of 61 toxigenic strains were isolated
including 54 strains (88.5%), with both tcdA and
tcdB genes positive and the remaining positive for
the tcdB gene alone. Results No binary toxin was
detected. The toxigenic strains were found to be susceptible to
vancomycin and metronidazole and exhibited high levels of resistance to
clindamycin, levofloxacin, erythromycin, and ciprofloxacin. The most
toxigenic C. difficile isolate was obtained from the
gastroenterology and infection ward. Additionally, 13 sequence types
(STs) were identified; ST-54 (32.8%), ST-3 (16.4%), ST-35 (13.1%), and
ST-37 (11.5%) were the most common types. Conclusions The results of the
present study indicate that CDI may be a common problem, and large-scale
multicenter studies are required to reveal the actual extent of the
burden of CDI in county level hospitals.
