Author/Authors :
Kazerouni Faranak نويسنده Department of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University, MC, Tehran , Bakhshi Mostafa نويسنده , Ebrahimi Firouz نويسنده Biology Research Center, Basic Sciences Faculty, Imam Hossein University (IHU), Tehran, IR Iran , Samadi Roghaieh نويسنده Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Zeynalian Hoda نويسنده Department of Laboratory Medicine, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , Rahimipour Ali نويسنده Department of Laboratory Medicine, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Production of antibodies in chickens (IgY) has significantly attracted the attention of scientists. Numerous publications have reported the use of IgY in diagnosis, therapy and prophylaxis. Production of antigen-specific antibodies in chicken can help treat and prevent infectious diseases. In this study, the safety of IgY (anti E. coli O157:H7) on the antioxidant system of mice was assessed. To that end, three different doses of IgY (0.9375, 1.875 and 3.75 g / kg) were administered through oral route to three treated groups and PBS to the control group. Each group consisted of 18 mice. Fourteen days after administration, blood samples were collected from the mice. Serum malondialdehyde (MDA) level and catalase, glutathione peroxidase and superoxide dismutase activity were measured using commercial kits. Oral administration of IgY against E.coli O157:H7 in doses of 0.9375, 1.875 and 3.75 g / kg caused no deaths and showed no toxic effects on mice. In this study, after 14 days of IgY administration, there were no significant changes in the activity of antioxidant enzymes (catalase, glutathione peroxidase and superoxide dismutase) and MDA serum level compared to the control group. This study’s findings revealed that oral administration of IgY against E. coli O157:H7 does not show any toxic effects and does not disturb the antioxidant system in mice. These findings could be indicative of safety of oral administration of IgY in mice.
