Author/Authors :
Farajollahi Alireza نويسنده Department of Medical Physics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , Pirayesh Islamian Jalil نويسنده Department of Medical Physics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , Sattarpour Zahra نويسنده Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , Baradaran Behzad نويسنده Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , Asghari Jafarabadi Mohammad نويسنده Department of Statistics and Epidemiology, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran , Khazeh Vahid نويسنده Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract :
Background: IMOD™, a selenium enriched extract of the plants Tanacetum
vulgare, Urtica dioica, and Rosa canina, has an excellent effect on oxidative stress. In
this study, we investigated the radioprotective effects of this immunomodulatory drug
on human peripheral blood lymphocytes.
Methods: Peripheral blood samples obtained from venipuncture of the brachial vein
were treated with IMOD™ (5, 10, 15, 20 ?l) for 30 min and Cobalt 60 ?-rays (0.25,
0.5, 1, 2 Gy) as the test groups and cultured with the control. We used the micronuclei
assay, cell death detection, and cell toxicity assay to analyze the treatment effects.
Results: The frequency of micronuclei were 1.66 (0 Gy), 5.33 (0.25 Gy), 9.67 (0.5
Gy), 17.67 (1 Gy), and 23.67 (2 Gy) in the irradiated lymphocytes (P < 0.001). The
percentage of micronuclei frequency reduced to 20%, 26.83%, 37.68%, 16%, and 20.47%
with IMOD™. Apoptosis and necrosis decreased significantly in the IMOD™ treated
groups (P < 0.05).
Conclusion: IMOD™ may protect these cells against ionizing radiation.
