Author/Authors :
Makhlough Atieh نويسنده , Maleki Iradj نويسنده Department of Internal Medicine, Mazandaran University of Medical Sciences, Sari, Iran Maleki Iradj , Haghshenas Mohammadreza نويسنده Department of Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran , Makhlough Marjan نويسنده Institute of Experimental Animal Research, Mazandaran
University of Medical Sciences, Sari, IR Iran , Mahdavi Mohammadreza نويسنده Thalassemia Research Center, Mazandaran University of
Medical Sciences, Sari, Iran , Davoodi Lotfollah نويسنده Infectious Disease Specialists, Antimicrobial Resistance
Research Center, Mazandaran University of Medical Sciences, Sari, IR
Iran , Mousavi Tahoora نويسنده PhD Candidate in Molecular and Cell Biology, Molecular and
Cell Biology Research Center, Student Research Committee, Mazandaran
University of Medical Sciences, Sari, IR Iran , Hasani-Mansoor Seyed-Hosein نويسنده Research Committee Center, Faculty of Medical Sciences,
Mazandaran University of Medical Sciences, Sari, Iran
Abstract :
Background Hemodialysis is one of the renal replacement therapies
in patients with end-stage renal failure. The current study aimed at
identifying the prevalence of hepatitis C virus (HCV) infection in
hemodialysis population, and comparing serological (enzyme-linked
immunosorbent assay) and molecular (polymerase chain reaction) methods
to detect HCV infection in North of Iran. Methods Serum samples from 162
patients undergoing chronic hemodialysis were collected in 2
hemodialysis units of Sari city (North of Iran). HCV RNAs were isolated
from samples using RIBO-prep nucleic acid extraction kit (AmpliSens®,
Russia). Total RNAs were extracted from samples and real-time polymerase
chain reaction (PCR) was performed using HCV-FRT PCR kit (AmpliSense,
Russia) according to the manufacturer’s instructions. Results In the
study, 7 (4.3%) cases were HCV-Ab positive and 155 (95.7%) HCV-Ab
negative. Additionally, 11 patients (6.8%) were HCV-PCR positive, while
151 (93.2%) were HCV-PCR negative. Among 11 HCV-PCR positive patients, 7
(63.6%) were HCV-Ab positive and 4 (36.4%) were HCV-ab negative. HCV-ab
test was not positive in any of the HCV-PCR negative patients.
Conclusions The results showed that the specificity of HCV-RNA detection
was significantly higher than that of the conventional HCV-ab test. The
gold standard test to confirm HCV positive should be PCR method.
