Author/Authors :
Sarbishegi Maryam نويسنده Department of Anatomy, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran , Khajavi Ozra نويسنده Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran , Alhagh Charkhat Gorgich Enam نويسنده Department of Histology, School of Medicine, Zahedan
University of Medical Sciences, Zahedan, IR Iran
Abstract :
Background Olive (Olea europaea), from the
Oleaseae family, is a very popular plant for its biological and
pharmacological characteristics. Olive tree derivatives possess
antioxidant, anti-inflammatory, antihyperlipidemic, and cardioprotective
effects. Objectives The current study aimed at evaluating the effect of
olive leaves extract (OLE) on sperm quality and antioxidant capacity in
testis of rats exposed to rotenone (ROT). Methods In the current
experimental study, 36 male Wistar rats were divided into 6 groups (n =
6). Control, Sham, ROT, and 3 ROTs treated with OLE (75, 150, and 300
mg/kg/day). ROT 3 mg/kg/48 hours was injected subcutaneously (s.c), and
vehicle or OLE was administrated by oral gavage for 30 days. At the end
of the study, animals were scarified and their testis tissue and
epididymis samples were removed and their sperm parameters (viability,
motility, and sperm count), total antioxidant capacity (TAC), and
malondialdehyde (MDA) level were measured. Results The results showed a
significant decrease in sperm parameters and TAC in the ROT group. MDA
level increased in ROT group compared to that of the control group. The
30 day administration of OLE significantly improved the sperm
qualitative parameters and TAC, and decreased the elevation of MDA in
the treated groups (P value < 0.05). Conclusions In conclusion,
the antioxidant effects of OLE significantly increased sperm quality and
TAC, and decreased MDA level in the testis of rats exposed to ROT. These
results revealed that OLE improved the sperm quality and suggested that
OLE may treat infertility.
