Author/Authors :
Haghjooy Javanmard Shaghayegh نويسنده Physiology Research Center , Jafari Elham نويسنده Tehran University of Medical Sciences , Vaseghi Golnaz نويسنده Physiology Reseach Center, Isfahan University of Medical Sciences, Isfahan , Dana Nasim نويسنده Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran , Tajaddini Mohammad Hasan نويسنده Department of Biochemistry, Applied Physiology Research
Center, Cardiovascular Research Institute, Isfahan University of
Medical Sciences, Isfahan, IR Iran , Abed Alireza نويسنده Department of Pharmacology, School of Medicine, Kashan
University of Medical Sciences, Kashan, IR Iran , Jamadi Maryam نويسنده Applied Physiology Research Center, Cardiovascular
Research Institute, Isfahan University of Medical Sciences, Isfahan,
IR Iran
Abstract :
Background Previous studies suggested that Cannabis
sativa has anti-cancer properties influencing tumor size and
metastasis properties. Microtubule associated proteins (MAPs) such as
tau binds to the microtubules and leads to cancer metastasis. Stathmin
is a cytosolic phosphor-protein which can induce the depolymerization of
microtubules in cancer cell lines, stathmin and tau
over expressions, and leads to reduced sensitivity to taxane. It seems
that C. Sativa can impress microtubule-associated
proteins existing in breast cancer stem cells. Objectives The effects of
the extract of Cannabis Sativa on
stathmin and tau relative gene expression were
studied. Methods Tau and stathmin
relative gene expressions were evaluated at different concentrations of
C. Sativa extract (final concentrations 0.1, 1, 10,
and 100 μg/mL) using real-time quantitative PCR. In order to understand
the mechanism, AM281 (Cannabinoid receptor 1) was administrated with
extract too. Results The extract exhibited significant differences (P
< 0.05 and P < 0.005 respectively) in
tau and stathmin relative gene
expression with the doses of 100 μg/mL compared to control group, the
mixture of antagonist AM281 and extract reversed the results.
Conclusions C. sativa extract dose-dependently
influences tau and stathmin
relative gene expressions, which may reduce cancer metastasis.
