Author/Authors :
Ramezani Amin نويسنده Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran , Hosseini Ahmad نويسنده Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , Eftekhar Ebrahim نويسنده Molecular Medicine Research Center, Department of Biochemistry, Hormozgan University of Medical Sciences, Bandar Abbas, Iran; , Seghatoleslam Atefeh نويسنده Department of Biochemistry-Recombinant Protein Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , Erfani Nasrollah نويسنده Cancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , Namavari Mohammad Mehdi نويسنده Razi Vaccine and Serum Research Institute, Shiraz Branch, Shiraz, Iran. Namavari Mohammad Mehdi , Amiri Ahmad نويسنده , Rashidi Mojtaba نويسنده Department of Biochemistry, Medical School, Shiraz
University of Medical Sciences, Shiraz, Iran , Fahmidehkar Mohammad Ali نويسنده Department of Clinical Biochemistry, Faculty of Medicine,
Rafsanjan University of Medical Sciences, Rafsanjan,
Iran , Fakher Shima نويسنده Department of Biochemistry, Medical School, Shiraz
University of Medical Sciences, Shiraz, Iran
Abstract :
Background Cancer is one of the major threatening factors of human
health worldwide. Unfortunately, chemotherapy, the powerful arm of
cancer therapy, is accompanied with many side effects, so alternative
treatments with greater specificities and fewer side effects are highly
required. Methods Human cancer cell lines including SW-742, HCT116,
HepG2, Hep2, MKN45 and LNcap were selected and the anti-cancer potential
of Cyrtopodion scabrum extract
(CsE) on their growth was studied. Vero cells were
used to study the potential cytotoxicity on the normal cells. Cell cycle
analysis and DNA fragmentation assay were also performed. Results
CsE was toxic (30% - 78%) to all the cell lines, with
the highest cytotoxicity on SW742, MKN45 and HepG2, respectively. A high
selectivity index (> 2) was observed for the extract on SW742 and
MKN45 cell lines. DNA laddering pattern, as well as a significant
increase in the number of the cells accumulated in sub-G1 and G2-phase
of the cell cycle compared to the control untreated cells, was also
observed. Conclusions CsE suppressed the human cancer
cells selectively and probably through apoptosis and G2 arrest
mechanism. It could suggest a promising alternative/complementary
treatment for cancer patients, especially those who suffer from
digestive tract cancer.
