Author/Authors :
Cangara M Husni نويسنده Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia , Hatta Mochammad نويسنده Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia , Miskad Upik A نويسنده Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia , Wahid Syarifuddin نويسنده Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Abstract :
Background: Angiogenesis is the process of new blood vessels formation that
contribute to tumor growth and metastasis. Endothelial cell-selective adhesion molecule
is one of the proteins that expresses in vascular endothelial cells. In vitro and animal
studies have shown involvement of this protein in physiological and pathological
angiogenesis. von Willebrand factor is a protein expressed by endothelial cells and
megakaryocytes that has a role in blood clotting processes. In the current study, we
investigate the expression of endothelial cell-selective adhesion molecule and von
Willebrand factor in carcinoma mammae specimens and explore their correlation
with tumor growth and metastasis.
Methods: We obtained 79 specimens from paraffin blocks of patients diagnosed
with invasive breast carcinoma of no special type. The slides from these specimens were
then stained with endothelial cell-selective adhesion molecule, von Willebrand factor,
and Ki-67 antibodies to assess vascular numbers and cell proliferation.
Results: We found a total of 31 (39%) low vascularity and 48 (61%) high vascularity
samples from endothelial cell-selective adhesion molecule staining. There were 34 (43%)
low vascularity and 45 (54%) high vascularity samples by von Willebrand factor
staining. There was a significant correlation of blood vessel numbers in the endothelial
cell-selective adhesion molecule-stained samples with tumor volume, metastasis to lymph
nodes, and proliferation cells. The von Willebrand factor-expressed samples only had
a significant correlation of vascular number with tumor volume.
Conclusion: Endothelial cell-selective adhesion molecule and von Willebrand
factor as the endothelial cell expressed proteins play a role in the angiogenesis process
of breast cancer. However, endothelial cell-selective adhesion molecule expression is
more consistent than von Willebrand factor in predicting the presence or absence of
metastatic breast cancer.
