Author/Authors :
Shahi Zahra نويسنده Department of Microbiology, College of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran , Bazi Saphora نويسنده Department of Biology, Faculty of Sciences, Payam-e-Noor University, Isfahan, Iran Bazi Saphora , Sohil Baigi Gelareh نويسنده Kermanshah University of Medical Sciences, Martyr Chamran Hospital Kangavar, IR Iran , Jahani Somayeh نويسنده Infectious Diseases and Tropical Medicine Research Center,Zahedan University of Medical Sciences,Zahedan,Iran , Mashhady Mohammad Amin نويسنده Department of Food Industry, Faculty of Agriculture, University of Zabol, Zabol, IR Iran , Sheykhzade Asadi Maryam نويسنده Department of Microbiology, Kerman Science and Research
Branch, Islamic Azad University, Kerman, Iran
Abstract :
[Background and Objectives]The aim of this study was to investigate the effect of ethanol extract of green tea on biofilm formation of Staphylococcus aureus.[Methods]Staphylococcus aureus samples were obtained from hospital sources and plant extracts; green tea was made using the rotary. The minimum inhibitory concentration was determined using the microdilution method. Inhibition of biofilm formation was determined by using a microplate.[Results]The results of this study showed that the greatest resistance was to the antibiotics penicillin (88.2%) and erythromycin (82.4%) and the least resistance to the antibiotic Amikacin (76/11%), respectively. The results of the effect of green tea extract showed the lowest inhibitory concentration (MIC) is equal to 0.3 milligrams per milliliter, which is a strain on the inhibitory concentration, and the highest concentration (MBC) MBC resistant Staphylococcus aureus equal to 20 milligrams per milliliter, which is controlled unidirectional. The results of green tea extract effects on biofilm showed that with increasing concentrations of less biofilm formation the biofilm formation has been much lower concentration of the extract.[Conclusions]The results of this study indicate good effects of green tea extract to inhibit biofilm formation of Staphylococcus aureus, which can be used to treat infections caused by these bacteria’s.
