Title of article
Role of HDM2 Gene in Radio-Sensitivity of Esophageal Cancer Cell Lines to Irradiation
Author/Authors
Mohammadi Mohsen نويسنده , Karami Hadi نويسنده Department of Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran Karami Hadi , Farajollahi Alireza نويسنده Department of Medical Physics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , Nejati-Koshki Kazem نويسنده Tabriz Genetic Analysis Center(TGAC) , Tabriz University of Medical Sciences, Tabriz, Iran , Pirayesh Islamian Jalil نويسنده Department of Medical Physics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , Oladghaffari Maryam نويسنده Department of Medical Physics, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Pages
6
From page
1
Abstract
[Background]Radio therapy plays an important role in controlling tumor growth in esophageal cancer patients.[Objectives]Our study provided comprehensive information about radio-sensitivity of Hdm2 gene in esophageal cancer cells in cancer cells.[Methods]The expression of Hdm2 gene in radiosensitive and radio-resistant esophageal squamous cell carcinoma TE1, TE8 and TE11 cell lines is studied. RNA extracted from each esophageal cancer cell, then Quantitative reverse transcription PCR (RT-qPCR) assay were used for measuring HDM2 gene expression. Finally, radiosensitivity was determined for every three esophageal cancer cell lines.[Results]It was found that Hdm2 gene significantly expressed in TE1, TE8 and TE11 cell lines by qRT-PCR analysis. Clonogenic assay showed that TE-11 was very sensitive to radiation relative to other cell lines.[Conclusions]Hdm2 protein binding to the P53 protein is a key regulator of cell cycle control and genome protection Cell cycle control, programmed cell death and response cells to radition play important roles.
Journal title
Astroparticle Physics
Serial Year
2017
Record number
2410420
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