Author/Authors :
Eslami Gita نويسنده , Nikmanesh Bahram نويسنده Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran , Samadi Roghayeh نويسنده Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , Ghalavand Zohreh نويسنده Iranian Tissue Bank & Research Center, Tehran University of Medical Science, Tehran, Iran. , Esmaeili Benvidi Mozhgan نويسنده Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , Nodeh Farahani Narges نويسنده Children’s Medical Center, Pediatrics Center of
Excellence, Tehran, IR Iran , Yasini Maryam نويسنده Department of Microbiology and Immunology, Kashan University of Medical Sciences, Kashan, Iran
Abstract :
[Background]The aim of the present study was to investigate biofilm formation among Methicillin-resistant Staphylococcus aureus (MRSA) isolated from children referring to a pediatric hospital in Tehran.[Methods]In total, 98 MRSA isolates were collected from children referring to a pediatric hospital during 2014 - 2015. All the isolates were confirmed to be MRSA using PCR amplification of the mecA gene. The antibiotic susceptibility patterns of the isolates were determined using the Kirby-Bauer disk-diffusion and E-test methods. In order to assess the ability of biofilm formation among the isolates, Congo red agar (CRA) and Microtiter Plate (Mtp) methods were used.[Results]All the isolates were found to be susceptible to linezolid and vancomycin and, likewise, the majority was susceptible to minocycline and rifampicin. CRA and Mtp methods showed that 81.6% and 63.3% of the MRSA isolates, respectively, were biofilm producers.[Conclusions]The early identification of S. aureus and detection of biofilm formation by the Mtp method are essential steps towards the prevention of the most serious nosocomial infections.
