Author/Authors :
Mukhtar Nadia نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , Yaqub Tahir نويسنده Department of Microbiology, University of Veterinary and
Animal Sciences, Lahore, Pakistan , Yasmin Farkhanda نويسنده Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan , Khan Idrees نويسنده Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan , Shahzad Wasim نويسنده Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan , Saeed Hashmi Abu نويسنده Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan , Tahir Zarfishan نويسنده Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan , Umar Sajid نويسنده Department of Pathobiology, PMAS Arid Agriculture University Rawalpindi, Pakistan
Abstract :
[Background]Dengue is one of the main health problems worldwide with dramatic increases in reported cases in the last few decades. Now, dengue is a major threat to half of the world’s population and approximately 50 million infections occur every year.[Objectives]The major aim of this study was to screen out different synthetic antiviral compounds against dengue virus. As treatments used for dengue virus infections are nonspecific and effectless, it is necessary to find specific antiviral compounds that may be helpful in the treatment of dengue fever. In cell culture models, thiazolides are active against anaerobic bacteria, protozoa, and a range of viruses.[Methods]Samples from positive dengue fever patients were included in the study. For dengue serotype-2, serum samples were further confirmed by serotyping protocol.[Results]After toxicological analysis, a sharp significant antiviral response was shown by compounds A and B decreasing the viral titer of serotype 2 as 46% and 53%, respectively. A greater antiviral activity was indicated by compound “B” against NS3 gene as compared to compound “A”. Against NS3 gene, a comparable antiviral activity was observed for compounds “A” and “B”.[Conclusions]From our results, it can be concluded that compounds A and B (Thiazolides) as dengue antiviral drugs can reduce viral load in patients if their inhibitory effects are reproduced in vivo. To identify the specific active ingredients, further studies are required by using various techniques to determine the efficacy of these compounds in animal model.
