Author/Authors :
Toka Ozer Turkan نويسنده Department of Medical Microbiology, Konya Hospital, Konya 42003, Turkiye , Karagoz Alper نويسنده Molecular Microbiology Research and Application Laboratory, Refik Saydam National Public Health Agency, Ankara 06100, Turkiye , Yalcin Gozde نويسنده Biotechnology Institute, Ankara University, Ankara 06110, Turkiye , A. Andac Cenk نويسنده Department of Medicinal Chemistry, School of Pharmacy, Istinye University, Istanbul 34010, Turkiye
Abstract :
[Background]Production of extended-spectrum β-lactamase (ESBL) is one of the antibiotic resistance strategies in the bacteria. Extended spectrum β-lactamase producing Escherichia coli (E. coli) infections alarmingly increased in recent years in Turkey.[Objectives]The current study aimed at determining antibiotic resistance and genotypic profiles of ESBL-positive E. coli isolates.[Methods]Forty-five ESBL-positive E. coli species were isolated from a variety of units both at the Mevlana University Foundation Hospital and the Mevlana University Medical Center at Konya province in Turkey from December 2013 to December 2014. Antibiotic resistance profile was determined by the Kirby-Bauer disk diffusion method. Genotypic profile was determined by pulsed-field gel electrophoresis (PFGE).[Results]The rate of ESBL production in E. coli strains was 13.1%. The isolates were highly resistant to penicillins, cephalosporins, and monobactams, while very low resistant to carbapenems. Four PFGE profiles were identified: profile A (2%), profile B (2%), profile C (67%), and profile D (29%). Profile C, the most commonly identified profile, possessed 6 subprofiles (profiles C1 - C6) with more than 85% clonal similarity; Profile C2 was the commonest identified subprofile of profile C (27%).[Conclusions]Extended spectrum β-lactamase producing E. coli strains were highly resistant to β-lactam antibiotics.
