Author/Authors :
Sharifi Asghar نويسنده Assistant Professor in Educational Managment, Tehran Science and Research Branch, Tehran, Iran. , Manzouri Leila نويسنده Department of Community Medicine, School of Medicine, Isfahan University of Medical Science, Isfahan , Ghalamfarsa Ghasem نويسنده Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , Khoramrooz Seyed Sajjad نويسنده Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IR Iran , Askarinia Marzieh نويسنده Student Research Committee, Yasuj University of Medical Sciences, Yasuj, IR Iran , Ghaedi Mehrorang نويسنده Chemistry Department, Yasouj University, Yasouj 75918-74831, IR Iran , Jannesar Ramin نويسنده Department of Molecular Microbiology, Dena pathobiology Laboratory, Yasouj, IR Iran , Sadri Farzad نويسنده Young Researchers and Elite Club, Yasouj Branch, Islamic Azad University, Yasouj, IR Iran , Abdolmajid Khosravani Seyed نويسنده Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, IR Iran
Abstract :
[Background]The antibiotic resistance of bacteria has increased in the last decade. The mecA gene plays an important role in the pathogenicity of Methicillin-Resistant Staphylococcus aureus (MRSA) by increasing antibiotics resistance. Recent studies have indicated that nanotechnology, as an antimicrobial agent, has had promising results.[Objectives]The present study was conducted to determine the effect of Cu-BPDCA-Ty on antibacterial activity and mecA gene expression in clinical and standard strains of MRSA.[Methods]The phenotypic tests were used to identify MRSA strain and confirmed with molecular detection of mecA gene. Synthesized Cu-BPDCA-Ty was confirmed with different techniques such as XRD and SEM analysis. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by the micro broth dilution method. Real time PCR was used to investigate gene expression. Pta gene was considered as an endogenous control for normalization. Data were analyzed using one sample t test and paired t test in the SPSS software Version 22.[Results]The findings indicated that the MIC and the MBC of Cu-BPDCA-Ty against the standard and clinical strains of MRSA were 0.5 mg/mL, 0.8 mg/mL, 0.46 ± 0.08 mg/mL, and 0.7 ± 0.1 mg/mL, respectively. Analysis of the real- time PCR indicated that all treated groups with Cu-BPDCA- Ty showed a significant decrease in the expression of the mecA gene compared to the control group (P < 0.05).[Conclusions]Cu-BPDCA-Ty had an antibacterial effect on MRSA and induced downregulation of expression of the mecA gene.
