Title of article :
Frequency of Helicobacter pylori hopQI, hopQII and sabA Genes Among Iranian Patients with Gastroduodenal Diseases
Author/Authors :
Shirzad Hedayatollah نويسنده Department of Immonology, Medical Plants Research Center, Shahr-e-Kord University of Medical Sciences, Shahr-e-Kord, Iran , Bagheri Nader نويسنده Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran , Shahi Heshmat Heshmat نويسنده Medical Genetics Department, National Institute of Genetic Engineering and Biotechnology (NIGEB). , Zamanzad Behnam نويسنده Medical Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran , Khashei Reza نويسنده Department of Bacteriology and Virology, School of
Medicine, Shiraz University of Medical Sciences, Shiraz, IR
Iran , Sedarat Zahra نويسنده Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran , Sadeghiani Marzieh نويسنده Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract :
[Background]Helicobacter pylori has been recognized as the most common pathogen of human gastroduodenal tract and it has been suggested that adhesins, including HopQ and SabA, are associated with the organism’s virulence.[Objectives]The current study aimed at determining the frequency of hopQI, hopQII, and sabA genes among H. pylori isolates from patients with gastroduodenal disorders in Shahrekord, Iran.[Methods]Gastric corpus samples were obtained from 150 symptomatic patients admitted to the endoscopy unit at gastroenterology clinic. After DNA extraction from all corpus samples, H. pylori molecular confirmation and genotyping was performed by the polymerase chain reaction (PCR), using specific primers for glmM, 16SrRNA and hopQ, sabA genes, respectively.[Results]The hopQI, hopQII, and sabA genes were found in 74 (49.3%), 59 (39.3%), and 43 (28.7%) cases, respectively. The hopQI gene was detected in 75% of patients with gastric cancer (GC), 42.4% with chronic gastritis (CG), and 57.4% with peptic ulcer disease (PUD). The hopQII among patients with GC, CG, and PUD was also detected in 50%, 38.8%, and 39.3%, respectively. Moreover, sabA was diagnosed in 50% of patients with GC, 29.4% with CG, and 26.2% with PUD.[Conclusions]No significant association was observed between hopQI, hopQII, and sabA genes with clinical outcomes.
Journal title :
Astroparticle Physics
