Author/Authors :
Samiei Leila نويسنده Ornamental Plant Department, Reasearch Center for Plant Sciences, Ferdowsi University of Mashhad. , Aghdaei Maedeh نويسنده PhD Student, Department of Horticultural Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran , Vessal Saeed Reza نويسنده Assistant Professor, Department of Legume Research, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad Iran
Abstract :
In vitro microbial contaminations are the most serious problems in
plant tissue culture. Initial attempts for micropropagation of Dionysia
tapetodes, a beautiful alpine cushion plant from north-east Iran, led to
100% explants contamination. Hence, the present study was conducted to
develop an efficient surface sterilization protocol for in vitro culture of
field collected D. tapetodes using combinations of different disinfection
agents including sodium hypochlorite (NaOCl), ethanol and mercuric
chloride (HgCl2) in different concentrations and exposure times. The
most effective sterilization treatment with 91.6% healthy explants was
the application of 0.1% mercuric chloride for 4 min. However, mercuric
chloride in higher concentration (0.2%) was toxic to explants tissues and
resulted in 41.66% necrosis. Moreover, the results indicated that in spite
of being a general disinfectant in tissue culture studies, NaOCl was not as
effective as mercuric chloride in elimination of microbial contaminations
and led to 75% total infection when used in concentration of 3% for 15
min. The current experiment represented a potential method for disinfection
of D. tapetodes and provided aseptic and clean explants for future in vitro
culture studies.
