Title of article :
Effect of Methyl Jasmonate and Sucrose on Endogenous Non-structural Carbohydrates in Petals and Leaves of Cut ‘First Red’ Roses (Rosa hybrida L.)
Author/Authors :
Foukaraki Sofia G. نويسنده Plant Science Laboratory - Cranfield University, Bedfordshire , Cook Balwinder نويسنده Plant Science Laboratory - Cranfield University, Bedfordshire , Papadimitriou Michael D. نويسنده School ofAgriculture, Technological Educational Institute of Crete, Heraklion, Crete , Terry Leon A. نويسنده Plant Science Laboratory - Cranfield University, Bedfordshire
Abstract :
Effects of exogenously applied methyl jasmonate (MeJA) and
sucrose on vase life and non-structural carbohydrate concentration in
petals and leaves of cut ‘First Red’ roses were investigated. Roses were
placed in sealed plastic containers and received MeJA vapour treatment
(0.1 ?L MeJA L-1) for 24 h at 20°C. Flowers were then placed in individual
bottles containing 0 or 2% (v/v) sucrose solution. Flower petals and the
two uppermost five-leaflet leaves were detached on days 0, d 5 and d 10
of vase life. Samples were individually snap-frozen in liquid nitrogen and
freeze-dried. Non-structural carbohydrates were extracted and quantified
using standard HPLC coupled to evaporative light scattering detection.
The MeJA vapour treatment enhanced vase life of flower and foliage of
‘First Red’ roses. Significant differences were observed between foliage
life of cut ‘First Red’ roses that were treated with MeJA and sucrose, but
not for flower life. Rose stems treated with MeJA in the absence of
sucrose had an extended vase life compared to roses treated with 2%
sucrose alone (14.0 vs. 12.8 days of vase life). Sucrose and myo-inositol,
and to a lesser extent glucose concentrations in petals of cut roses
decreased during vase life, even when flowers were supplied with 2%
sucrose. Concomitant to this, fructose levels in petals increased during
vase life. Neither sucrose nor MeJA had a significant effect on any of the
sugars measured in petals of cut roses. In contrast, significant differences
were apparent for all sugars measured in leaves that were treated with
MeJA and sucrose solutions. The combination of MeJA and 2% sucrose
solution sharply increased endogenous sucrose concentration in leaves,
but the opposite was shown in the absence of 2% sucrose. Sucrose
treatment alone did not consistently alter endogenous sucrose concentration.
Interactions between MeJA and sucrose on sugar metabolism are discussed.
Journal title :
Astroparticle Physics
