Author/Authors :
Rostami Nejad Mohammad نويسنده Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran , Chizari Zahra نويسنده Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran , Nazemalhosseini Mojarad Ehsan نويسنده Gastroenterology and Liver Diseases Research Center - Shahid Beheshti University of Medical Sciences , Keshavarz Akbar نويسنده Department of Parasitology - Shahid Beheshti University of Medical Sciences , Taghipour Nilofare نويسنده Department of Parasitology - Shahid Beheshti University of Medical Sciences , Mahmoudi Lamuki Reza نويسنده Gastroenterology and Liver Diseases Research Center - Shahid Beheshti University of Medical Sciences , Salehi Atefeh نويسنده Gastroenterology and Liver Diseases Research Center - Shahid Beheshti University of Medical Sciences , Haghighi Ali نويسنده Department of Parasitology - Shahid Beheshti University of Medical Sciences
Abstract :
Cryptosporidium is a globally distributed protozoan parasite and one of the most common causes of infection and diarrhea in humans and cattle. The aim of the present study was to determine the species of Cryptosporidium among cattle with diarrhea by a nested PCR-RFLP technique at Cryptosporidium oocyst wall protein (COWP). Fecal samples from 158 calves aged 1-20 weeks were collected from 10 dairy farms in Qazvin province, Iran. Initial identification of Cryptosporidium was carried out by Zeihl-Neelsen acid-fast staining method of stool samples. DNA was extracted from 26 (16.45 %) positive microscopically samples and Cryptosporidium genotypes were determined. Cryptosporidium parvum were identified in 80.8% of the positive samples and, Cryptosporidium andersoni in 19.2%. In conclusion the use of COWP primers could be sensitive enough to conduct a routine detection study. The nested PCR method using the COWP gene sequence can be an alternative diagnostic method to identify infected with Cryptosporidium and its genetic diversity.
