Author/Authors :
PINGILI, RAVINDRABABU Department of Pharmacology - KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India , VEMULAPALLI, SRIDHAR Department of Pharmacology - KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India , SANDEEP MULLAPUDI, SURYA Department of Pharmacology - KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India , KONDRU, SIVARAMAKRISHNA Department of Pharmacology - KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India , CHUNDURU, NAGABHUSHNAM Department of Pharmacology - KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India , KILARU, NAVEENBABU Department of Pharmacology - KVSR Siddhartha College of Pharmaceutical Sciences, Vijayawada, Andhra Pradesh, India
Abstract :
Since long back, herbal medicines have been the highly-esteemed source of medicine; therefore, they
have become a growing part of modern, high-tech medicine. Zephyranthes candida (ZC) has been
mentioned in the Indian System of Traditional Medicine for the treatment of diabetes mellitus. The
objective of this study was to evaluate the anti-diabetic activity of methanolic leaf extract of Zephyranthes
candida (MLZ) and its different fractions in healthy and Streptozotocin (STZ)-induced diabetic rats.
Healthy wistar and STZ-induced diabetic rats were treated orally with MLZ (100, 200 and 400 mg/kg) and
glipizide (5 mg/kg) for 21 consecutive days. Blood samples were collected from the retro orbital plexus on
1st, 8th, 15th and 21st day. In another study, STZ-induced diabetic rats were treated with glipizide (5
mg/kg), fraction I [hexane: ethyl acetate (1:1)], fraction II [chloroform: methanol (1:1)] and fraction III
[chloroform: methanol (2:8)]. Blood glucose levels and lipid profiles were determined using ERBA,
semiautoanalyzer. The methanolic extract was further analyzed for phytochemical analysis. MLZ (100,
200 and 400 mg/kg) showed a significant reduction in blood glucose levels which were comparable to
that of the standard anti-diabetic drug, glipizide. The total cholesterol, triglycerides and low density
lipoproteins levels were significantly reduced by MLZ in diabetic rats. All the three fractions are also
reduced the blood glucose levels after single oral administration (p<0.01). In phytochemical analysis,
MLZ showed the presence of flavonoids, glycosides and alkaloids. The present study results indicated
that Zephyranthes Candida possess anti-diabetic and lipid lowering effects may be due to the antioxidant
activity of flavonoids or alkaloids. Further studies are needed to elucidate the structures and to evaluate
the exact mechanism of anti-diabetic action of the active components.
Keywords :
Zephyranthes Candida , Diabetes Mellitus , Hypoglycemic , Antihyperlipidemic , Antihyperglycemic
