Salvianolic acid B improves insulin secretion from interleukin 1B-treated rat pancreatic islets: The role of PI3K-Akt signaling

Background and Objective: Oxidative stress induced by proinflammatory cytokines such as IL-1B plays a major role in B-cell destruction in diabetes type 1. Salvianolic acid B (Sal B) is a polyphenolic compound with antioxidant and protective effects. Thus, objective of this study was to assess the protection exerted by Sal B on isolated rat islets exposed to IL-1B and to investigate an underlying mechanism in vitro. Materials and Methods: Isolation of pancreatic islets was done by using the collagenase digestion method. Isolated rat islets were divided into 6 groups including: 1. control, 2. interleukin-1B treated, 3 and 4. interleukin-1B treated+ Sal B, 5 and 6. interleukin-1B treated+ Sal B+ PKB and PI3K inhibitors. Interleukin-1B (1 U/ml) was used to induce cytotoxicity after pretreatment with two doses of Sal B (50 ?M and 100 ?M) and application of each inhibitors was before Sal B. Results: IL-1B significantly decreased insulin secretion from isolated islets. Pretreatment with Sal B ameliorated the effect of IL-1B on glucose stimulated insulin secretion in a concentration dependent manner. Inhibitors of PKB and PI3K both abolished these improving effect of Sal B. Conclusion: Sal B that has antioxidant, anti inflammatory and anti apoptotic properties, provided resistance to pancreatic B-cell dysfunction from cytokine in part via PI3K/Akt pathway. The findings represent that it is a promising agent for prevention of B-cell dysfunction in type 1 diabetes.