Author/Authors :
Kwon, Jung-Taek Laboratory of Toxicology - College of Veterinary Medicine - Seoul 151-742, Korea , Jiang, Hu-Lin Laboratory of Toxicology - College of Veterinary Medicine - Seoul 151-742, Korea - school of Pharmacy - China Pharmaceutical University - Nanjing 210009, China , Minai-Tehrani, Arash Laboratory of Toxicology - College of Veterinary Medicine - Seoul 151-742, Korea , Woo, Chang Gyu Nanotechnology and Thermal Processing Laboratory - School of Mechanical and Aerospace Engineering - Seoul 151-742, Korea , Choi, Mansoo Nanotechnology and Thermal Processing Laboratory - School of Mechanical and Aerospace Engineering - Seoul 151-742, Korea , Cho, Chong-Su Research Institute for Agriculture and Life Sciences - Seoul National University - Seoul 151-742, Korea , Kim, Yeon-Soo Indang Institute of Molecular Biology - Inje University - Seoul 100-032, Korea , Cho, Myung-Haing Department of Nanofusion Technology - Graduate School of Convergence Science and Technology - Seoul National University - Suwon 443-270, Korea - Advanced Institute of Convergence Technology - Seoul National University - Suwon 443-270, Korea
Abstract :
Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for the
improvement of low transfection efficiency of chitosan. The present study aims to test the pulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposed to aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHIg- PEI complexes for 30 min during the development of our nose-only exposure chamber (NOEC) system. CHI-g-PEI-mediated aerosol delivery demonstrated 15.65% enhancement of the fluorescence intensity. Compared to PEI, CHI-g-PEI showed no significant pulmonary toxicity. In summary, using CHI-g-PEI is safe and shows high transfection in aerosol gene delivery to animals, and enhanced efficiency was achieved through our aerosol gene delivery system. Therefore, CHI-g-PEI and this system would be applicable to future study for aerosol gene therapy.
Keywords :
Aerosol delivery , Chitosan , Gene carrier , Polyethylenimine , Pulmonary toxicity
