Author/Authors :
Niknejad, Farhad Laboratory Sciences Research Center - Golestan University of Medical Sciences, Gorgan , Moshfegh, Mahsa Department of Pharmaceutical Biotechnology - Faculty of Pharmacy and Biotechnology Research Center -Tehran University of Medical Sciences, Tehran , Najafzadeh, Mohammad Javad Department of Parasitology and Mycology and Cancer Molecular Pathology Research Center - Ghaem Hospital - School of Medicine - Mashhad University of Medical Sciences , Houbraken, Jos Department of Applied and Industrial Mycology - CBS-KNAW Fungal Biodiversity Centre - Utrecht - The Netherlands , Rezaei, Shahla Department of Pharmaceutical Biotechnology - Faculty of Pharmacy and Biotechnology Research Center -Tehran University of Medical Sciences, Tehran , Zarrini, Gholamreza Department of Animal Sciences - Faculty of Natural Sciences - University of Tabriz, Tabriz , Faramarzi, Mohammad Ali Department of Pharmaceutical Biotechnology - Faculty of Pharmacy and Biotechnology Research Center -Tehran University of Medical Sciences, Tehran , Nafissi-Varcheh, Nastaran Department of Pharmaceutical Biotechnology - School of Pharmacy and Protein Technology Research Center - Shahid Beheshti University of Medical Sciences, Tehran
Abstract :
Four halotolerant fungal isolates originating from the saltwater Lake Urmia in Iran were selected during a screening program for salt resistance and α-amylase activity. The isolates were identified based on sequencing the ITS region and a part of the β-tubulin gene, as Penicillium chrysogenum (isolate U1; CBS 132820), Fusarium incarnatum (isolate U2; CBS 132821), and Penicillium polonicum (isolate U3; CBS 132822, and isolate U4; CBS 132823). The growth of these isolates was determined by measuring the colony diameter and mycelia dry weight in Sabouraud dextrose agar and yeast nitrogen base medium supplemented with NaCl, KCl, and LiCl. Isolate U4 showed a growth up in 15% NaCl and U1 was the only isolate that could grow in 20% KCl. None of the strains grew in a media containing LiCl. The salt supplemented
medium did not increase the size of colony diameter in all isolates (p > 0.05). The ability of the selected isolates for amylase production was quantitatively tested and showed that P. polonicum isolate U4 was the most potent producer of amylase with a yield of 260.9 U/L after 60 h, whereas P. polonicum isolate U3 was the lowest one with a production level of 97.9 U/L after 48 h. P. polonicum isolate U4 could be a suitable candidate for production of amylase on an industrial scale after optimization.
Keywords :
Isolation , Amylase , Halotolerant , Fungi , Hypersaline environments
