Title of article :
Cycloartane Triterpenoids from Euphorbia Macrostegia with their Cytotoxicity against MDA-MB48 and MCF-7 Cancer Cell Lines
Author/Authors :
Baniadam, Somayeh Isfahan Pharmaceutical Sciences Research Center - Isfahan University of Medical Sciences, Isfahan , Rahiminejad, Mohammad Reza Department of Biology - Faculty of Science - University of Isfahan , Ghannadian, Mustafa Department of Pharmacognosy - School of Pharmacy - Isfahan University of Medical Sciences , Saeidi, Hojjatollah Department of Biology - Faculty of Science - University of Isfahan , Ayatollahi, Abdul Majid Phytochemistry Research Center,Department of Pharmacognosy - School of Pharmacy - Shahid Beheshti University of Medical Sciences, Tehran , Aghaei, Mahmoud Department of Clinical Biochemistry - School of Pharmacy - Isfahan University of Medical Sciences, Isfahan
Abstract :
The dried plant was extracted with dichloromethane and after defatting with hexane,
transferred repeatedly on silica columns using dichloromethane-hexane and ethyl acetatehexane as mobile phases. Finally the fractions were purified by high performance liquid chromatography using a Pack-Sil column and hexane: Ethyl acetate as mobile phase. The structures of the isolated compounds included: cycloart-25-ene-3β, 24-diol (1), cycloart-23(Z)- ene-3β, 25-diol (2), cycloart-23(E)-ene-3β, 25-diol (3), and 24-methylene-cycloart-3β-ol (4) were elucidated by 13C- and 1H-NMR as well as IR and by the aid of mass fragmentation pattern and comparing with the literature. The biological effects of the compounds were done by the MTT assay on two different cancer cell lines including MDA-MB48 and MCF-7. Among these compounds, cycloart-23(E)-ene-3β,25-diol (3) was the most active compound on MDAMB468 cell line (LD50 = 2.05 μgmL− 1 ) and cycloart-23(Z)-ene-3β, 25-diol (2) was the most active compound on MCF-7 cell line (LD50 = 5.4 μgmL− 1).
Keywords :
Euphorboa macrostegia , Cycloartane , Cytotoxicity , MDA-MB468 , MCF-7
Journal title :
Astroparticle Physics
