Author/Authors :
Bamdad, Shahram Shiraz University of Medical Sciences (SUMS), Shiraz, Iran , Bamdad, Mina Department of Developmental Biology - Islamic Azad University, Shiraz, Iran , Khanlari, Mahsa Shiraz Molecular Pathology Research Center - Dr Daneshbod Lab, Shiraz, Iran , Daneshbod, Yahya Shiraz Molecular Pathology Research Center - Dr Daneshbod Lab, Shiraz, Iran , Khademi, Behzad Shiraz University of Medical Sciences (SUMS), Shiraz, Iran
Abstract :
In this research study, to investigate teratogenic effect of intravitreal injection of bevacizumab
in pregnant rat model, twenty seven female Wistar rats were inseminated. Pregnant rats were
divided into 6 groups (three groups as case and three as control groups). Each case and control
groups were divided according to the day of intravitreal injections (day 2, 10 and 18). Rats in
the case groups received 4 μL intravitreal injection of bevacizumab and the control groups
received the same volume of distilled water. The tail and umbilical cord length in case groups
1, 2, and 3 did not display any significant differences compared to their control groups. The
fetal weight was significantly lower in the case groups 1 (p >0.001) and 2 (p> 0.001) compared
to their control groups. Furthermore, the placental weight was only lower in the case group
1 (p >0.001). Case group 2 had a shorter crown rump length in comparison with its control
group (p=0.029). Morphological investigations showed two abnormal cases of gastroschisis
in group 1 and a case of a cleft in the skull in one of the rats in case group 2. The results show
that intravitreal bevacizumab has developmental effect when administered in the early stages of
pregnancy; but it is safe when administered in the last week of pregnancy in rats.
Keywords :
Teratogenicity , Rat , Peregnancy , Itravitreal Injection , Avastin , Anti VEGF , Animal Model
