Title of article :
The Evaluation and Comparing of Cytotoxic Effects of Ferula gummosa Gum, Scutellaria lindbergii, Kelussia odoratissima and Artemisia kopetdaghensis Extracts on ACHN Cell Line
Author/Authors :
Hosseini, Azar Pharmacological Research Center of Medicinal Plants - School of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Bakhtiari, Elham Eye Research Center - School of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Khajavi Rad, Abolfazl Department of Physiology - School of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Shahraki, Samira Department of Physiology - School of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Mousavi, Hadi Medical Toxicology Research Center - School of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Havakhah, Shahrzad Department of Physiology - School of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Amiri, Mohammad Sadegh Department of Biology - Payeme Noor University, Tehran, Iran
Abstract :
Renal cell carcinoma (RCC) is one of most fatal cancers. In most patients it is resistant
to chemotherapy. Ferula gummosa gum, Scutellaria lindbergii, Kelussia odoratissima, and
Artemisia kopetdaghensis are herbs about which there are some cytotoxic activity reports. In
this study, cytotoxic and apoptotic activity of these four extracts on RCC cell line (ACHN)
were evaluated and compared (ACHN) cells were treated with different concentrations of
herbal extracts (15-500 μg/mL). Cell proliferation was determined after 24, 48, and 72 h. by
MTT assay. Apoptotic cells were determined using PI staining of DNA fragmentation by flow
cytometry. Cell viability decreased with all herbal extracts in ACHN cells by 24, 48, and 72
h. as compared with control. Extracts induced a sub-G1 peak in flow cytometry histogram of
treated cells indicating apoptotic cell death is involved in extracts induced-toxicity. Results
imply that four herbal extracts inhibit the growth of ACHN cells as a concentration- and timedependent
manner. Also, results show that apoptosis is proposed as the possible mechanism
of action. So, four herbal extracts could be considered as good anticancer agents in RCC after
further studies.
Keywords :
Artemisia kopetdaghensis , Kelussia odoratissima , Scutellaria lindbergii , Ferula gummosa gum , Cytotoxicity , Apoptosis
