Title of article
Microbial Hydroxylation of 16α, 17α-Epoxyprogesterone by Penicillium Decumbens
Author/Authors
Mao, Shuhong Key Laboratory of Industrial Fermentation Microbiology - Ministry of Education -Tianjin University of Science & Technology, Tianjin, China , Wang, Xuerong Tianjin Key Laboratory of Industrial Microbiology - College of Biotechnology - Tianjin University of Science & Technology, Tianjin, China , Ge, Zhijiang Tianjin Key Laboratory of Industrial Microbiology - College of Biotechnology - Tianjin University of Science & Technology, Tianjin, China , Su, An Department of Chemistry and Chemical Biology - Rensselaer Polytechnic Institute, Troy, NY, USA , Zhang, Lixia Tianjin Key Laboratory of Industrial Microbiology - College of Biotechnology - Tianjin University of Science & Technology, Tianjin, China , Li, Yanqing Tianjin Key Laboratory of Industrial Microbiology - College of Biotechnology - Tianjin University of Science & Technology, Tianjin, China , Liu, Xiaoguang Key Laboratory of Industrial Fermentation Microbiology - Ministry of Education -Tianjin University of Science & Technology, Tianjin, China , Lu, Fuping Key Laboratory of Industrial Fermentation Microbiology - Ministry of Education -Tianjin University of Science & Technology, Tianjin, China
Pages
6
From page
1161
To page
1166
Abstract
Microbial transformation has been successfully applied in the production of steroid
intermediates with therapeutic use and commercial value in pharmaceutical industry due to its
high regio- and stereo-selectivity. As such, it is still important to screen microbial strains with
novel activity or more efficient abilities in the development of the commercial steroid industry.
Biotransformation of steroid: 16α, 17α-epoxyprogesterone (1). using Penicilliumdecumbens as
biocatalyst was investigated and selective hydroxylation of 1 was observed. The products were
separated by silica gel column chromatography, and the structure determination was performed
by MS, NMR, and X-ray crystallography. Biotransformation of 1 afforded 7β-hydroxy-16α,
17α-epoxyprogesterone (2). and 7β,11α-dihydroxy-16α,17α- epoxyprogesterone (3). The two
novel metabolic products 2 and 3 were reported for the first time. Moreover, the identified
C7β- and C11-αhydroxylation is a novel reaction of microbial transformation of steroids by
P.decumbens.
Keywords
Hydroxylation , 17α-Epoxyprogesterone , 16α , Penicillium decumbens , Biotransformation , Steroid
Journal title
Astroparticle Physics
Serial Year
2017
Record number
2416459
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