Author/Authors :
Canadanovic-Brunet, Jasna , Vulic, Jelena University of Novi Sad - Faculty of Technology - Bulevar Cara Lazara 1, Novi Sad, Serbia , Cebovic, Tatjana University of Novi Sad - Faculty of Medicine - Hajduk Veljkova 3, Novi Sad, Serbia , Cetkovica, Gordana University of Novi Sad - Faculty of Technology - Bulevar Cara Lazara 1, Novi Sad, Serbia , Canadanovic, Vladimir University of Novi Sad - Faculty of Medicine - Hajduk Veljkova 3, Novi Sad, Serbia , Djilas, Sonja University of Novi Sad - Faculty of Technology - Bulevar Cara Lazara 1, Novi Sad, Serbia , Saponjac, Vesna Tumbas University of Novi Sad - Faculty of Technology - Bulevar Cara Lazara 1, Novi Sad, Serbia
Abstract :
Raspberry waste, obtained from two cultivars, Meeker (ERM) and Willamette (ERW) was
subjected to evaluation antioxidants and antitumour activities as a potential source of phenolics.
Some phenolic acids and flavonoids were identified and quantified by HPLC. Antioxidant
activity was tested by measuring ability to scavenge DPPH• and •OH by ESR spectroscopy.
IC50
DPPH• varied from 0.67 for ERM to 0.54 mg/mL for ERW, while IC50
•OH values varied from
3.73 for ERM to 1.23 mg/mL for ERW. Cytotoxic activity was investigated using in vivo
model of Ehrlich Ascite carcinoma cells (EAC) in mice. Pretreatment with extracts exhibited
potent cytotoxic activity against EAC cells (up to 60%) and both extracts inhibited the tumour
growth. Activity of xanthine oxidase (XOD) was extremely increased in pretreated animals,
while the activity of enzyme complex glutathione reductase (GR) and glutathione peroxidase
(GSHPx) was significantly increased. This study suggests that raspberry pomace could be used
as nutraceutic resource and functional food ingredient.
Keywords :
antioxidant enzymes , antioxidant activity , HPLC , raspberry pomace
