Author/Authors :
Sannigrahi, Santanu St. Peter’s Institute of Pharmaceutical Sciences, Warangal, Andhra Pradesh, India , Kanti Mazuderb,Upal Department of Pharmaceutical Technology - Jadavpur University - Kolkata, India , Kumar Pal, Dilip Department of Pharmaceutical Chemistry - Seemanta Institute of Pharmaceutical Sciences, Jharpokharia, India , Parida, Sambit St. Peter’s Institute of Pharmaceutical Sciences, Warangal, Andhra Pradesh, India , Jain, Sourabh St. Peter’s Institute of Pharmaceutical Sciences, Warangal, Andhra Pradesh, India
Abstract :
The antioxidant potential of crude methanol extract (CE) as well as chloroform (CF), ethyl acetate (EF) and n-butanol (NF) soluble fractions of Enhydra fluctuans Lour, which is widely used in indigenous system of medicine for different purposes, were studied. The antioxidant potential of extract/different fractions were evaluated using different in vitro antioxidant models. In addition, total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical, nitric oxide, superoxide anion, hydroxyl radical scavenging activities and reductive power of crude extracted its different fractions were determined. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (179.7 ± 18.23 μg / mg pyrocatechol equivalent). This fraction was found more effective than crude extract and other fractions in all the above mentioned assays
Keywords :
Antioxidant activity , Enhydra fluctuans , Different fractions , Free radical scavenging activity , Flavonoids
