Author/Authors :
Bagheri, Fereshteh Pharmaceutical Sciences Research Center - School of Pharmacy - Kermanshah University of Medical Sciences, Kermanshah, Iran , Tahvilian, Reza Pharmaceutical Sciences Research Center - School of Pharmacy - Kermanshah University of Medical Sciences, Kermanshah, Iran , Karimib, Naser Department of Biology - Faculty of Sciences - Razi University, Kermanshah, Iran , Chalabi, Maryam Department of Immunology - School of Medicine - Medical Biology Research Center - Kermanshah University of Medical Sciences, Iran , Azami, Mahsa Pharmaceutical Sciences Research Center - School of Pharmacy - Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract :
The objective of this research was in-vitro germination and callus induction of Onosma
bulbotrichum (O. bulbotrichum) as a medicinal herb which belongs to Boraginaceae family.
For germination, the seeds were cultured on growth regulator-free MS medium and for callus
induction, seeds were sown on modified MS medium containing different concentrations of
kinetin (kn)- Indole-3-acetic acid (IAA) and kn- 2,4-D (2,4-dichlorophenoxyacetic acid),
respectively. The plates were maintained in the dark at growth chamber. After 7 days seed
germination on hormone-free medium and after 10 days callus initiation on modified medium in
the presence of hormones was occurred. The maximum pigmented callus (100%) was observed
on modified MS medium with a combination of 0.2 mg.L-1 IAA + 2.10 mg.L-1 kn. Shikonin
determination was performed by HPLC method. In addition, total hydroxynaphtoquinons
as polyphenols in sum of callus and culture medium were measured by spectrophotometric
method and revealed that total naphtoquinones content at IAA was more than 2, 4-D
Keywords :
HPLC , 2,4-Dichlorophenoxyacetic acid , Indole-3-acetic acid , Callus , Germination , Onosma bulbotrichom
