Author/Authors :
Hassanpour, Mehdi Department of Clinical Biochemistry and Laboratory Medicine - Tabriz University of Medical Sciences, Tabriz, Iran , Cheraghi, Omid Department of Biochemistry - Faculty of Biological Sciences - Tarbiat Modares University, Tehran, Iran , Brazvan, Belal Stem Cell Research Center - Tabriz University of Medical Science, Tabriz, Iran , Hiradfar, Amirataollah Pediatric Health Research Center - Tabriz University of Medical Sciences, Tabriz, Iran , Aghamohammadzadeh, Nasser Endocrine and Metabolism Section - Department of Internal Medicine - Tabriz University of Medical Sciences, Tabriz, Iran , Rahbarghazi, Reza Stem Cell Research Center - Tabriz University of Medical Science, Tabriz, Iran , Nouri, Mohammad Stem Cell Research Center - Tabriz University of Medical Science, Tabriz, Iran
Abstract :
By virtue of lifestyle change, incidence of diabetes mellitus type 2 is increasingly being
raised with different up-surging pathologies. It was showed that endothelial progenitor cells
(EPCs) were disqualified in neo-angiogenesis induction. Besides, to an aborted differentiation
property, malfunctioned paracrine activities worsen off vascular abnormality. Nano-scaled
exosomes play essential roles in reciprocal cell-cell crosstalk via bioactive molecules. To address
the effect of diabetic serum on exosome secretion capacity, EPCs were exposed to diabetic
condition for seven days. In addition to in-vitro tubulogenesis, migration and LDL uptake
assessment, exosome release capacity, and expression profiles of three genes participating in
exosome kinetics, including CD63, Alix and Rab27a, revealed by Real-time PCR method. Data
showed diabetic sera not only abolished the in-vitro tubulogenesis, migration and LDL uptake
properties but also decreased exosome release and expression of related genes. This study sheds
lights on the adverse effect of diabetic condition on exosome kinetics in EPCs.
Keywords :
Kinetics , Exosome , Diabetes , Endothelial progenitor cells
