Author/Authors :
Saeidi, Yasaman Department of Microbiology - Tonekabon Branch Islamic Azad University, Tonekabon, Iran , Pournajaf, Abazar Department of Microbiology - Tonekabon Branch Islamic Azad University, Tonekabon, Iran , Gholami, Mehrdad Department of Microbiology - Tonekabon Branch Islamic Azad University, Tonekabon, Iran , Hasannejad-Bibalan, Meysam Department of Microbiology - Tonekabon Branch Islamic Azad University, Tonekabon, Iran , Yaghoubi, Sajad Division of Microbiology - Department of Pathobiology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , Khodabandeh, Mahmoud Department of Pediatric Infectious Disease - Faculty of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Emadi5, Behzad Department of Microbiology - School of Medicine - Iran University of Medical Sciences - International campus, Tehran, Iran , Ferdosi-Shahandashti, Elaheh Department of Medical Biotechnology - Faculty of Medicine - Babol University of Medical Sciences, Babol, Iran , Rajabnia, Ramazan Infectious Diseases and Tropical Medicine Research Center - Babol University of Medical Sciences, Babol, Iran
Abstract :
Background: Helicobacter pylori (H. pylori or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis,
ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for H. pylori, and some of them appear to be related
to more severe clinical consequences of the infection. The present study was conducted to determine cagA, vacA, cagE, iceA1, oipA, and
iceA2 genes in H. pylori strains isolated from gastroduodenal patients, who referred to Shariati hospital in Tehran, Iran.
Methods: Gastric biopsy specimens were collected during endoscopy from patients, who referred to the Shariati hospital in Tehran,
Iran during January and November 2015. After isolation of H. pylori from the biopsy culture, genomic DNA was extracted and subsequently
used to identify H. pylori and virulence genes using specific primers.
Results: The isolation rate of H. pylori strains was 65.7% (169/257). The frequency of cagA, vacA, cagE, iceA1, oipA, and iceA2 was
143 (% 84.6), 169 (100%), 131 (77.5%), 97 (57.3%), 89 (52.6%), and 72 (42.6%), respectively.
Conclusion: In this study, a significant difference was observed between investigated genes and strains isolated from PUD and GC
patients (p<0.05).
Keywords :
Duodenal ulcers , Multiplex PCR , Virulence factors , Iran , Helicobacter pylori
