Author/Authors :
Ezati, Tohid Department of Plant Biotechnology - National Institute for Genetic Engineering and Biotechnology, Tehran, Iran , Marefatjoo, Mohammad Javad Agriculture and Natural Resources Department - Islamic Azad University, Karaj Branch , Haghbeen, Kamahldin Department of Plant Biotechnology - National Institute for Genetic Engineering and Biotechnology, Tehran, Iran , Ahmmadkhaniha, Reza Pharmaceutical Sciences Research Center - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Arnebia pulchra (Willd. ex Roem. & Schult.) Edm. is an endangered, perennial member of Boraginaceae
family. Spectrophotometric and LC-Mass analysis confirmed the existence of shikalkin derivatives in its root
skin and polyphenolic compounds in the inner part of its root. Studies on the regeneration of A. pulchra from its
callus resulted in successful callus induction in the root explants excised from the young plantlets on MS
medium amended with 2,4-D (1 mg.l-1) and kinetin (0.5 mg.l-1) at 25 °C in darkness. The highest frequency of
shooting (15 shoots per callus) was achieved for the calli sub-cultured on MS medium supplemented with 2,4-D
(0.1 mg.l-1) and kinetin (2 mg.l-1) in 2 weeks. The concentration and type of auxin were found critical for
successful rooting. The best rooting conditions with a frequency rate of 80% were obtained for the regenerates
which were sub-cultured on half-strength MS medium supplemented with IBA (1 mg.l-1), NAA (1 mg.l-1) and
kinetin (0.2 mg.l-1). The regeneration method developed in this work provides a basis for germplasm
conservation of A. pulchra.
Keywords :
Arnebia pulchra , Callus , Rooting , Shikalkin , Shooting
