Highly Sensitive Voltammetric Sensors based on Modified Gold Nanoparticles Electrode for Determination of Nepafenac in Bulk Powder, Pharmaceutical Dosage Form and Blood Serum

This article pronounces differential pulse and square wave voltammetric techniques for determination of Nepafenac in pharmaceutical dosage form and human serum. The proposed techniques were established on electro-oxidation of nepafenac at two electrodes, the first electrode is carbon paste modified with carbon multiwalled nanotubes and plated with Gold nanoparticles (GN-CMWNT-CPE) in 0.1 M HCl and the second electrode is the carbon paste modified with multiwalled carbon nanotubes and 1-n-butyl-3- methylpyridinium hexafluorophosphate ion crystal and plated with Gold nanoparticles particles (GN-CMWNT-BMH-CPE) in 0.1 M H2SO4. The peak current concentration relationship was rectilinear over the range 3.33-36.63 and 6.67- 46.69 μg/mL for (GN-CMWNT-BMH-CPE) in DPV and SQW modes, respectively, and 6.67-33.35 μg/mL and 6.67-40.02 μg/mL for (GN-CMWNT- -CPE) in both DPV and SQW modes. LOD was 0.557 and 0.929 μg/mL for (GN-CMWNT-BMH-CPE) in DPV and SQW modes, respectively, 1.213 and 0.806 μg/mL for (GN-CMWNT- -CPE) in both DPV and SQW modes. Experimentations were optimized using glassy electrode. Precision and accuracy of were checked with good repeatability and reproducibility. The methods were applied successfully to the pharmaceutical dosage form and human serum.